丙戊酸钠对幼鼠代谢的影响及肝毒性的试验探讨

Effects of valproate on metabolism in rats and liver toxicity tests

目的:通过观察丙戊酸钠(VPA)作用后的Wistar幼鼠的肝功能、瘦素、胰岛素的变化分析对其影响因素。方法:选用SPF级3周龄雄性Wistar大鼠36只,平均体重48g。并随机分为两组(每组23只),空白对照组:经口灌胃等量蒸馏水;VPA组:经口灌胃VPA300mg/(kg·d),每周2次;3个月后检测体重变化、瘦素、胰岛素、血清ALT、血清AST、血清GST、肝MDA、肝GSH以及肝GST。结果:与空白对照组比较,VPA组AST、肝GST、瘦素、胰岛素显著升高(P<0.01),体重显著增加(P<0.05),肝GSH含量显著降低(P<0.01),而血清ASL、血清GST、肝MDA则均无统计学意义(P>0.05)。结论:长期应用VPA会造成肝毒性及心血管系统潜在危险。

Objective： To investigate the effects of valproate sodium （VPA） on hepatic injury and leptin,insulin via the observations of biochemical indicators in immature rat. Methods： Sixty 3-week-old male Wistar rats were randomly divided into two groups： experimental groups （VPA group） and negative control group （23 for each group）.After 3 months,test the weight , Serum leptin, Serum insulin, Serum ALT, Serum AST, Serum GST,liver MDA, Serum GSH and the Serum GST. Results： Serum leptin , insulin, AST and liver GST levels in both experimental groups （VPA group） were remarkably higher than the of the negative control group（P〈0.01）,but liver GSH levels in both experimental groups（VPA group） were remarkably lower. Conclusion： VPA can cause the potential dangerous in liver and cardiovascular system.