口腔癌肿瘤浸润淋巴细胞对人舌癌裸鼠移植瘤的生长抑制作用

Inhibition effects of tumor infiltrating lymphocytes from oral cancer on nude mice transplanted tumor established with human tongue carcinoma cell lines

目的探讨在体外具有强大细胞毒活性的口腔癌浸润淋巴细胞在体内的抑瘤效果以及化疗药物环磷酰胺（ｃｙｃｌｏｐｈｏｓｐｈａｍｉｄｅ，Ｃｙ）与肿瘤浸润淋巴细胞（ｔｕｍｏｒｉｎｆｉｌｔｒａｔｉｏｎｌｙｍｐｈｏｃｙｔｅ，ＴＩＬ）联合应用治疗口腔癌的可能性。方法将舌鳞癌细胞系Ｔｃａ８１１３注入裸鼠背部皮下建立移植瘤模型，取培养４周的ＴＩＬ联合低剂量Ｃｙ局部注射，观察肿瘤体积变化及肿瘤生长情况。结果①ＴＩＬ＋ｒＩＬ２和ＴＩＬ＋ｒＩＬ２＋Ｃｙ组在３周内抑瘤率较高（分别为：８６１％±０４％和９７７％±０６％），３周后抑瘤作用减弱；②ＴＩＬ＋ｒＩＬ２＋Ｃｙ组抑瘤率较ＴＩＬ＋ｒＩＬ２组高（第８周抑瘤率分别为：２００％±１４％和７５５％±２５％，Ｐ＜００１）。结论口腔癌ＴＩＬ在应用后３周内具较强抑瘤作用，３周后减弱；

Objective To observe the in vivo inhibition effects on nude mice transplanted tumor with tumor infiltrating lymphocytes (TILs) isolated from primary mass of oral cancer.Methods Established the transplanted tumor model of human tongue cancer at the back of BALB/C nude mice with subcutaneous injection of squamous cell carcinoma lines Tca 8113. TILs isolated from patients with Oral cancer, combining with low dose of cyclophosphamide (Cy: 50mg/kg), were locally injected into the peripheral site of tumor. The inhibition rate (IR) was calculated by the volume of tumor mass from the 1 st to the 8th week, and the weights at the 8th week after tumor dissection. Results ①Group TIL+rIL 2 and group TIL+rIL 2+Cy both exerted a strong inhibition effect on the transplanted tumor within three weeks. The Inhibition rate (IR) were 86 1%±0 4% and 97.7%±0.6% respectively at the 3rd week, while 32.1%±0.3% and 80.6%±0.3% at the 8th week. ②TIL+rIL 2+Cy expressed a stronger effect and a longer inhibition time. The IR of TIL+rIL 2 and TIL+rIL 2+Cy were 20.0%±1.4% and 75.5%±2.5% respeetively at the 8th week ( P <0 01).Conclusion This study demonstrated that TILs from patients with oral carcer possess strong in vivo inhibition on nude mice transplanted tumor, and low dose of cyclophasophamide can enhance the inhibition effect and prolong the inhibition time.