TGF_β·VEGF及PCNA在胚胎肠管的表达及其生物学意义

Expression of TGFβ·VEGF and PCNA on the gut of rats withdifferent embryonic days and its biological significance

目的：探讨各种细胞因子在肠管发生及实质细胞分化中的调控机理。方法：通过免疫组织化学及组织形态学分析方法，对转化生长因子（ＴＧＦβ），血管内皮生长因子（ＶＥＧＦ）和增殖细胞核抗原（ＰＣＮＡ）在胚胎期１３～１８ｄ之胎鼠肠管的表达进行研究。结果：①在胎鼠肠管ＴＧＦβ、ＶＥＧＦ、ＰＣＮＡ均有表达；②ＴＧＦβ在Ｅ１３时，在上皮及间充质呈高表达；③ＰＣＮＡＥ１８时，在上皮呈高表达；④ＶＥＧＦ先在间充质中高表达而后则有上皮表达高峰。结论：ＴＧＦβ对实质细胞增殖，具有抑制及促进分化作用。ＶＥＧＦ在促血管生成作用的同时。

Objective:In order to study modulating mechanism of different cellular factors in genesis and perenchymal cell differentiation of gut.Methods:The expression of TGFβ,VEGF and PCNA on the gut of rats with different embryonic days were investigated by the immunohistochemical and morphological analystic methods.Results:①TGFβ,VEGF and PCNA had positive expression on the gut;②TGFβ expressed highly on the epithelial and interstitial of gut in E13;③PCNA expressed highly around E18;④VEGF expressed highly on the interstitial around E13E14,then expressed highly on the epithelial in the E18.Conclusion:TGFβ and VEGF could play an important role in the regulation of cell proliferation and differentiation.