摘要
目的:研究人参总皂甙对心肌缺血和再灌注损伤的保护作用及浓度效应关系。方法:①11条杂种犬分二组,建立体外循环。人参总皂甙(GS)组心停搏液中加入GS20mg/L,测定心肌力学和血液动力学指标;取右室心肌标本电镜观察,线粒体记分。②30只大耳白兔分成5组,建立离体左心转流作功模型。GS组于心停搏液中加入GS20、40、80、160mg/L,测定心肌力学和血液动力学指标,同时取左心耳标本测定心肌内钙含量。结果:犬体外循环转流前后,兔离体左心转流作功前后,GS组与对照组比较,心肌力学指标±dp/dtmax,血流动力学指标CI(心脏指数),线粒体记分,心肌内钙含量,GS组均优于对照组(P<0.001),且GS80mg/L组优于GS20、40mg/L组(P<0.001),而GS160mg/L组上述指标均低于对照组(P<0.05)。结论:①GS20~80mg/L,对心肌缺血和再灌注损伤有明显的保护作用,且随浓度增加,作用增强。GS160mg/L,作用相反。
Objective:To study the effects of ginsenosides (GS) on myocardial ischemia and reperfusion injury,and concentrationeffect relationship.Methods:①Eleven mongral dogs were divided into 2 groups randomly and cardiopulmonary bypass was estabilished.In GS group,20 mg/L of GS was added into the cardioplegia.The indexes of myocardial dynamics and hemodynamics were recorded at 15 min after aortic unclamping.The samples were taken from the right ventricle and mitochodria were scored under electron microscope at 60 min of aortic crossclamp and 15 min after unclamping of aorta.②Thirty rabbits were divided into 5 groups randomly,and then isolated working hearts underwent 60 min normothermic ischemia by aortic crossclamping.In GS group 20,40,80,160 mg/L of GS were added into the cardioplegia respectively..As mentioned above,the parameters (at 25 min of aortic unclamping) and the myocardial samples were obtained.In addition the contents of calcium in the cardium were measured.Results:Before and after the dogs′cardiopulmonary bypass and the isolated rabbit hearts′working were established,the data of myocardial dynamics (±dp/dtmax),hemodynamics (CI),mitochodria score and the myocardial calcium content in GS group were better than those in control group (P<0001),and those in GS 80 mg/L group better than those in 20 and 40 mg/L groups,whereas all the data mentioned above in GS 160 mg/L group were lower than those in control gorup.Conclusion:①GS 20 ̄80 mg/L has significantly protective effect on the myocardial ischemia and reperfusion injury,and the effects increase when the concentration of GS increases;whereas the effects are converse when the concentration of GS was 160 mg/L.②GS has the calcium channel block effect.
出处
《白求恩医科大学学报》
CSCD
1998年第3期254-256,共3页
Journal of Norman Bethune University of Medical Science
关键词
人参总皂甙
心肌缺血
再灌注损伤
保护作用
ginsenosides (GS)
myocardial ischemia and reperfusion injury
concentrationeffect relationship