隧道式拖线术结合九一丹对大鼠体表瘘管的治疗作用

Combination of Thread-Dragging Therapy and "Jiuyi Dan" in Treating Fistula in Rats

目的:观察隧道式拖线术结合九一丹治疗大鼠体表瘘管的愈合情况,并探讨其作用机制与安全性。方法:72只体表瘘管大鼠随机分为治疗组(隧道式拖线术+九一丹)、对照组(瘘管切开+九一丹)和空白组。观察术后各组大鼠瘘管的愈合情况,分阶段检测各组大鼠碱性成纤维细胞生长因子(bFGF)含量及测定大鼠血清尿素氮(BUN)、血清肌酐(Scr)和血清丙氨酸转氨酶(ALT)值。结果:治疗组平均治愈时间为(16.25±1.96)d,对照组平均治愈时间为(23.75±0.96)d,两组比较有显著性差异(P<0.01)。治疗组治疗后第5天大鼠血bFGF含量明显高于对照组和空白组(P<0.05);术后第7天治疗组和对照组的大鼠血bFGF含量均明显高于空白组(P<0.05,P<0.01),但治疗组和对照组之间无显著性差异。在第7天和14天对照组的血清BUN、Scr均高于治疗组和空白组(P<0.05,P<0.01),在愈合时各组间血清BUN、Scr和ALT值无明显差异。结论:采用隧道式拖线法配合使用九一丹可以提高内源性bFGF含量,加速创面愈合;且隧道式拖线术为非开放性创面,九一丹吸收量相对小,对肝肾功能无明显不良影响,安全性好。

To evaluate the effects of ＂JiuYiDan＂ in combination of thread-dragging through fistula method on rat with fistula. Methods： A total of 72 Male SD rats with fistula were randomly divided into study group treated with thread-dragging through fistula method plus ＂JiuYiDan＂, the control group treated with fistulectomy plus ＂JiuYiDan＂ and the blank group without any therapy. Change of bFGF content in blood are mesured in 3,5, 7,9,14and cure day, respectively. And hepatic and renal function index （include BUN, Scr, ALT） are measured in 7,14 and cure day, respectively. Results： There was significant difference in the average healing time between the study group and the control group with the former was （16.25± 1.96） d and the latter （23.75 ± 0.96） d. in the 5th day, the content of bFGF in the study group was significant higher than those of the control group and blank group（P 〈 0.05）. In the 7th day, both the content of bFGF in the study group and control group were significant higher than that of blank group （P 〈 0.05 或 P 〈0.01 ） ,without significant difference between them. In theTth and 14 th day, the serum BUN and Scr of control group was significant higher than those of the study group or blank group （ P 〈 0.05或 P 〈 0.01 ）. But there is no distinction of the above index among the three groups in cure day. Conclusion ： Using ＂Jiuyi Dan＂ in thread-dragging through fistula therapy can increase the bFGF content and accelerate the healing of the wound. Moreover, the absorption of ＂Jiuyi Dan＂ is relatively little due to the non-open wound of the therapy, thus having no evident influence on hepatic and renal function. Therefore, the therapy is quite safe in practice.