温州3044例献血者戊型肝炎病毒血症及基因分型的研究

3044 blood donors Hepatitis E viremia and genotyping study in Wenzhou

目的了解温州市无偿献血者戊型肝炎病毒血症及基因分型的情况。方法对2008年2～10月浙江省温州市中心血站的无偿献血者进行整群抽样,检测抗HEV IgG以及IgM抗体,并对抗HEV IgM阳性的标本进行RT-PCR检测,经测序后分析其基因型及序列同源性。结果3044例无偿献血者中,HEV IgG阳性率为33.28%(1013/3044),IgM阳性率为0.92%(28/3044)。对28例HEV IgM阳性标本进行PCR检测共发现3例核酸阳性,核酸总体阳性率为0.1%,其中2例为基因Ⅳ型感染,1例为基因Ⅰ型感染。结论无偿献血者中存在一定比例的HEV病毒携带者,有必要对现有血液筛查策略下输血途径传播戊肝的风险进行评估。

Objective To investigate hepatitis E virus （HEV） viremia and genotypies in voluntary blood donors in Wenzhou. Methods Cluster sampling strategy was used to sample all blood donors from February to October in 2008 in Wenzhou Central Blood Bank. Blood was tested for IgG and IgM antibodies against HEV. Reverse transcriptase - poly- merase chain reaction （RT- PCR） and sequencing were applied to detect its genotype and sequence homology in HEV IgM - positive specimen. Results The prevalence of anti - HEV IgG in 3044 cases of blood donors was 33.28%. The positive rate of IgM was 0.92%. Nucleic acids were found in three cases through PCR amplification in all 28 cases of HEV IgM positive samples. The total HEV RNA positive rate was 0.1% , of which two cases were genotype IV, one case was genotype I ~ Conclusions There is a certain percentage of HEV carriers in voluntary blood donors. It is necessary to evaluate the risk of blood transfusion transmitted HEV under the current blood screening strategy.