酶联免疫法与液质联用法测定动物源性食品中沙丁胺醇和克伦特罗

Research of qualitative ELISA and HPLC-MS/MS for sabutamol and clenbuterol in animal-based food

目的:建立高灵敏度的动物源性食品中沙丁胺醇和克伦特罗的测定方法。方法:用定量酶联免疫法对动物源性食品进行初筛,然后对阳性样品用高效液相色谱-质谱联用法进行确证。结果:应用酶联免疫法对测定动物源食品中沙丁胺醇及克伦特罗线性范围为0.06~4.0μg/L,相关系数-0.996^-0.999。样品添加试验沙丁胺醇的回收率为71.2%~87.2%,克伦特罗回收率为70.4%~85.2%。高效液相色谱-质谱联用法测定动物源性食品中沙丁胺醇及克伦特罗含量,方法的线性范围均为0.1~10.0μg/L,线性相关系数分别为0.9998及0.9992,定量检出限分别为0.2及0.5μg/kg,回收率分别为89.9%~96.7%及88.4%~96.2%。结论:酶联免疫法灵敏度高,样品处理简单,适合大批量样品中沙丁胺醇和克伦特罗半定量筛选,成本较低;高效液相-质谱联用法灵敏度高,抗干扰能力强,适合对样品中沙丁胺醇和克伦特罗残留进行检测及结构确证。

Objective：To establish the high sensitive method for sabutamol and clenbuterol in animal -based food. Methods： The qualitative ELISA was based on the specific immuno -chemical reactions between antigen and antibody. ELISA was used for screening sabutamol and clenbuterol in animal - based food and the positive results were proved with HPLC - MS/MS, Results： The linear range of the ELISA method was 0. 06 - 4. 0 μg/L, and the correlation coefficient of the standard curve - 0. 996 -0. 999. The recoveries of sabutamol for the spiked animal - based food ranged from 71.2% to 87.2% and of clenbuterol ranged from 70.4% to 85.2% with an average recovery of 81.7% and 80. 1%. The linear range of the HPLC - MS/MS method was 0. 1 10.0 μg/L with linear correlation coefficient of 0. 9998 for sabutamol and of 0. 9992 for clenbuterol. The limit of detection was 0. 2 and 0. 5 μg/kg. The recoveries of sabutamol for the spiked samples ranged from 89. 9% to 96. 7% and those of clenbuterol ranged from 88.4% to 96. 2%. Conclusion：The ELISA method is sensitive with simple sample extracting, and suitable for detection for trace sabutamol and clenbuterol of mounts of sample with lower cost. The HPLC - MS/MS method is suitable for sabutamol and clenbuterol detection and structure proof with high sensitivity and anti -interference.