氢化物发生-原子荧光法测定茶饮料中的硒

Determination of selenium in tea by hydride generator atomic fluorescence spectrophotometry

目的：探讨应用氢化物发生-原子荧光法测定茶饮料中硒含量的方法和技术。方法：样品经硝酸-高氯酸消化后用原子荧光光度计测定。结果：在实验条件下该方法线性范围为0.0-2.0μg/L,相关系数r=0.9999,检出限为0.044μg/L,低、中、高3个浓度相对标准偏差分别为5.19%、1.97%、0.87%,回收率为94.9%-116.3%。结论：该方法精密度及回收率高,操作简单,是测定硒的可靠方法。

Objective： This article mainly discusses the method and technology of determining selenium in tea by using atomic fluorescence photometers. Methods：Samples were digested with nitric acid and perchloric acid, and then determined by atomic fluorescence photometers. Results：Under the conditions proposed, the linear range for the determination of selenium was found to be 0.0 - 2. 0 μg/L with the correlation coefficient of 0. 9999. The detection limits was 0. 044 μg/L and the value of relative standard deviation was 5.19%, 1.97% ,0. 87% in low, mid and high three different kinds of density respectively. The recovery rate of spiking standard for total selenium was in the range of 94. 9% - 116. 3 %. Conclusion：This method is good in precision and recovery. The detection of selenium in tea by atomic fluorescence spectrometry is practicable.