火焰原子吸收法测定全血中铁、锌、钙

Flame atomic absorption spectrometry for determination of Fe,Zn,Ca in whole blood

目的：建立火焰原子吸收法直接测定血液中铁、锌、钙的方法。方法：抽取静脉血,用全血稀释液稀释样品,以硝酸镧为电离抑制剂,直接进样测定。结果：铁浓度在0.50-3.00μg/m l范围时,相关系数0.99992,加标回收率91.7%,检出限（3σ）0.006μg/m l;锌浓度在0.015-0.090μg/m l范围时,相关系数0.9993,加标回收率92.5%,检出限（3σ）0.015μg/m l;钙浓度在0.50-2.00μg/m l范围时,相关系数0.9994,加标回收率96.7%,检出限（3σ）0.006μg/m l。结论：方法简便快捷,测定结果准确可靠,适合于批量分析。

Objective： To establish a method, named flame atomic absorption spectrometry, to determine the content of the serum iron, zinc, calcium directly. Methods：Transfer the venous blood sampling, dilute with whole blood, and use the lanthanum nitrate as the ionization inhibitors, then, direct determination. Results： When the content of iron is in the range of 0. 50 - 3.00 μg/ml, related coefficient refers to 0. 99992, the recovery obtained is 91.7%, the detection limit （3 σ） is 0. 006 μg/ml; while the content of zinc is in the range of 0. 015 - 0. 090 μg/ml, related coefficient refers to 0. 9993, the recovery obtained is 92. 5%, the detection limit （3 or） is 0. 015 μg/ml; while the content of calcium is in the range of 0. 50 - 2.00 μg/ml, related coefficient refers to 0. 9994, the recovery obtained is 96.7%, the detection limit （ 3 or） is 0. 006 μg/ml. Conclusion：The method is convenient and rapid, and the results are accurate. So it is suitable for large quantity analysis.