3种方法提取血清中HBVDNA的比较

Comparison of three isolation methods for HBV DNA

目的:比较3种HBV DNA提取方法对PCR产物量的影响。方法:使用碱裂解法、直接煮沸法和裂解液煮沸法同时提取血清HBV DNA,抽提物作PCR后,产物行琼脂糖凝胶电泳,并对阳性扩增条带进行密度定量分析。结果:碱裂解法所得HBV DNA量高于其他两种方法。结论:碱裂解法提取HBV DNA简单、快速,值得临床与实验室推广。

Objective： To compare the recovery of HBV DNA by three different methods of template preparation. Methods： An Alkaline lysis method was used to isolate serum HBV DNA and compared with serum boiling method and boiling lysis method. Results：The HBV DNA quantitative values by PCR using the alkaline lysis were higher than those by the other methods. Conclusion ： The alkaline lysis method is simple, rapid and efficient for extraction of serum HBV DNA and suitable for HBV DNA quantitative PCR detection.