摘要
目的:比较3种HBV DNA提取方法对PCR产物量的影响。方法:使用碱裂解法、直接煮沸法和裂解液煮沸法同时提取血清HBV DNA,抽提物作PCR后,产物行琼脂糖凝胶电泳,并对阳性扩增条带进行密度定量分析。结果:碱裂解法所得HBV DNA量高于其他两种方法。结论:碱裂解法提取HBV DNA简单、快速,值得临床与实验室推广。
Objective: To compare the recovery of HBV DNA by three different methods of template preparation. Methods: An Alkaline lysis method was used to isolate serum HBV DNA and compared with serum boiling method and boiling lysis method. Results:The HBV DNA quantitative values by PCR using the alkaline lysis were higher than those by the other methods. Conclusion : The alkaline lysis method is simple, rapid and efficient for extraction of serum HBV DNA and suitable for HBV DNA quantitative PCR detection.
出处
《中国卫生检验杂志》
CAS
2009年第5期1058-1058,1084,共2页
Chinese Journal of Health Laboratory Technology