嘌呤核苷酸对海洛因依赖及戒断大鼠心肌酶学的影响

Effect of purine nucleotide on myocardial enzymes of heroin dependence and withdrawal rats

目的:通过心肌酶学测定研究海洛因依赖及戒断对大鼠心肌酶的影响及嘌呤核苷酸的干预效果。方法:建立海洛因依赖及戒断大鼠模型,80只建模成功的Wistar大鼠随机分成对照组(生理盐水)、海洛因组、戒断3 d组、戒断9 d组、核苷酸组(不给海洛因)、海洛因加核苷酸组(同时给药)、核苷酸3 d组及核苷酸9 d组(每组10只),检测各组大鼠血浆中肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)、羟丁酸脱氢酶(HBDH)及天门冬酸氨基转移酶(m-AST)的活性。结果:与对照组比较,海洛因组各种心肌酶活性无明显变化(P>0.05);戒断3 d组和戒断9 d组心肌酶CK、CK-MB、LDH、HBDH和m-AST活性均明显降低(P<0.01或P<0.05);核苷酸组、海洛因加核苷酸组各种酶活性变化差异无显著性(P>0.05);核苷酸3 d和9 d组CK、CK-MB、LDH和m-AST活性无明显变化(P>0.05),而核苷酸3 d组HBDH活性明显升高(P<0.05),核苷酸9 d组HBDH活性仍然升高明显(P<0.01)。结论:短期给予海洛因后即可对心肌产生损伤,并有一定的延迟性和持续性,且不能在停止给药后立即消除;补充嘌呤核苷酸后能够改善心肌细胞的功能状态,使心肌酶含量恢复或接近正常。

Objective To study the cardiac damage of the heroin dependence and withdrawal rats and the influence of myocardial enzymes by purine nucleotide. Methods The rat models of heroin dependence and withdrawal were set up. 80 Wistar rats were randomly divided into 8 groups （10 rats of each group）： control （C）, heroin administration group （H）, 3 d withdrawal group （W3）, 9 d withdrawal group （W9）, nucleotide group （N）, horoin and nucleotide group （HN）, nucleotide 3 d group （N3）, nucleotide 9 d group （N9）. The activities of total creatine kinase （CK）, the myocardial isoenzyme of creatine kinase （CK-MB）, lactate dehydrogenase （LDH）, a hydroxybutyrate dehydrogenase （HBDH） and m-aspartate aminotransferase （m-AST） in plasma were tested. Results Compared with control group, the activities of CK, CK-MB, LDH, HBDH and m-AST in 3 d withdrawal group and 9d withdrawal group were significantly lower （P〈0.01, P〈0.05）, while the activities of CK, CK-MB, LDH, HBDH and m-AST in heroin administration group, nucleotide group, heroin and nucleotide group didn＇ t change significantly （P〉0.05）. And compared with control group, the activities of HBDH in 3 d nucleotide group and 9 d nucleotide group increased significantly （P〈0. 05, P〈0.01）, and the activities of CK, CK-MB, LDH and m-AST were considered normal （P〉0. 05）. Conclusion The heroin has delayed and durative damage to the cardiac muscle, and can＇t eliminate immediately after stopping administration; purine nucleiotide can diminish this damage and maintain some plasma myocardial enzymes of heroin dependence and withdrawal rats in relatively steady condition, and improve the functional status of cardiac muscle cells.