超声联合超声造影剂对VEGF基因体外转染成纤维细胞转染率的影响

Effect of ultrasound combined with contrast agent on efficiency of VEGF gene transferring into fibroblasts in vitro

目的:观察诊断剂量超声联合超声造影剂对血管内皮细胞生长因子(VEGF)基因转染体外培养成纤维细胞转染率的影响。方法:将成纤维细胞分为单纯VEGF165质粒转染组、造影剂+VEGF165质粒转染组、超声+VEGF165质粒转染组[机械指数(MI)为1.6]和超声+造影剂+VEGF165质粒转染组(MI分别为1.2、1.4和1.6)。转染后在激光共聚焦显微镜下观察各组VEGF在成纤维细胞中的转染率。结果:超声+VEGF165质粒组、造影剂+VEGF165质粒组与单纯VEGF165质粒组转染率比较差异无显著性(P>0.05);超声+造影剂+VEGF165质粒组(MI 1.2、1.4、1.6)转染率增加,与单纯VEGF165质粒组转染率比较差异均有显著性(P<0.01);其中超声+造影剂+VEGF165质粒组(MI 1.4、1.6)转染率最高,但2组间比较差异无显著性(P>0.05);超声+造影剂+VEGF165质粒组(MI 1.4、1.6)与超声+造影剂+VEGF165质粒组(MI 1.2)比较差异也有显著性(P<0.05)。结论:联合超声造影剂诊断剂量的超声照射能提高VEGF基因的转染率,且随超声剂量增加转染率升高.

Objective To observe the efficiency of vascular endothelial cell growth factor （VEGF） gene transferring into fibroblasts in vitro by diagnostic ultrasound combined contrast agent. Methods The fibroblasts were divided into four groups： pure VEGF165 plasmid, contrast agent and VEGF165 piasmid, ultrasound plus VEGF165 plasmid （MI 1.6）, ultrasound plus contrast agent and VEGF165 plasmid （MI 1.2, 1.4, and 1.6）. The transfection efficiencies of the VEGF gene in the fibroblasts were observed under confocal microscopy respectively in different groups. Results There was no significant difference of transfection efficiency among ultrasound plus plasmid group, contrast agent plus plasmid group and pure plasmid group （P〉0.05）. The transfection efficiencies were improved significantly in the ultrasound plus contrast agent and VEGF plasmid group （MI 1.2, 1.4, and 1.6） compared with pure plasmid group （P〈0.01）. The transfection efficiencies were higher in ultrasound plus contrast agent and VEGF165 plasmid group （MI 1.4, 1.6）, but there was no significant difference between the two groups （P〉 0.05）; there were significant differences between ultrasound plus contrast agent and VEGF165 plasmid （MI1.4, 1.6）and ultrasound plus contrast agent and VEGF165 plasmid group （MI 1.2） （P〈 0.01）.Conclusion Ultrasound combined with contrast agent can improve the transfection efficiency of VEGF gene, and the transfection efficiency increases with the intensity of ultrasound.