舌鳞状细胞癌中人乳头瘤病毒感染

Human papillomavirus infection in lingual squamous cell carcinoma

目的为了解人乳头瘤病毒，尤其HPV16与舌鳞癌发生的关系。方法采用免疫组织化学技术和PCR技术，对40例福尔马林固定、石蜡包埋舌鳞状细胞癌术后标本进行人乳头瘤病毒感染相关性研究。结果12／38例舌鳞癌癌旁上皮细胞中发现不同程度HPV结构抗原表达，而癌细胞中未见表达。30／40例人乳头病毒公共引物PCR阳性；25／30例HPV16PCR阳性。结论人乳头瘤病毒，尤其HPV16与舌鳞癌的发生关系密切。

Objective To study the association of human papilloma virus with human lingual squamous cell carcinoma.Methods Immunohistochemistry and PCR were used to detect human papillomavirus infection in 40 cases of formalin fixed and paraffin - embedded lingual carcinomas. Results 12 cases of 38 lingual cancers was found different level expression of HPV structural antigen in the epithilia adjacent to carcinoma, but not in cancer cells by immunohistochemistry. 30cases of 40 lingual carcinomas was detected HPV genetic sequence by general primer - mediated PCR, in which 25 caseswas detected HPV16 DNA sequence by specific primer- mediated PCK. Conclusion Human papillomaviruses, especiallyHPV16, were involved in the carcinogenesis of lingual carcinoma.