颈复康颗粒对颈椎动静力失衡大鼠颈椎间盘细胞外基质胶原表达的影响

Effects of Jingfukang Granule on the Collagen Expression in Extracellular Matrix of Cervical Intervertebral Disc in Rats Induced by Unbalanced Dynamic and Static Forces

目的:探讨颈复康颗粒调节大鼠退变椎间盘组织细胞外基质中Ⅱ型胶原和X型胶原mRNA表达的作用。方法:Realtime RT-PCR法检测椎间盘组织细胞外基质中Ⅱ型胶原和X型胶原mRNA表达水平。结果:与正常对照组相比,模型组Ⅱ型胶原mRNA的表达下降,X型胶原mRNA的表达增高,差异有统计学意义(P<0.01);与模型组相比,颈复康颗粒和莫比可组Ⅱ型胶原mRNA的表达上调,但差异无统计学意义;X型胶原mRNA的表达下降,差异有统计学意义(P<0.01)。结论:颈复康颗粒可以上调椎间盘组织内Ⅱ型胶原mRNA、下调X型胶原mRNA的基因表达。

Objective：To investigate the functions of Jingfukang Granule in regulating the expressions of type Ⅱ and X collagen in extracellular matrix of degenerated cervical intervertebral disc in rats. Methods： The mRNA expressions of type Ⅱ and X collagen in extracellular matrix were measured with real time RT--PCR. Results： Compared with that in control group, the mRNA expression of type Ⅱ collagen was down--regulated, and type X collagen up--regulated in the model group （P〈0.01）. Compared with that of model group, the mRNA expression of type Ⅱ collagen was slightly up--regulated, and type X collagen significantly down regulated in rats treated with Jingfukang Granule plus Mobic （P〈0.01）. Conclusion：Jingfukang Granule can up--regulate the expression of type Ⅱ collagen and down--regulate type X collagen in cervical intervertebral disc.