黄芪注射液预处理对大鼠脊髓缺血再灌注损伤脊髓ICAM-1表达的影响

Preconditioning of Huangqi Injection on the Expression of Intercellular Adhesion Molecule-1 in Spinal Cord Ischemia-reperfusion Injury in Rats

目的:探讨黄芪注射液对脊髓缺血再灌注损伤的保护治疗作用及其机制。方法:采用肾下腹主动脉阻断法,建立脊髓缺血再灌注模型。缺血组阻断腹主动脉30min后开放灌注。黄芪注射液治疗组静注黄芪注射12ml/kg30min后阻断腹主动脉,阻断腹主动脉30min后开放再灌注。术后进行神经功能评分,观察脊髓标本的病理改变,免疫组化染色ICAM-1表达。结果:神经功能评分治疗组在各时间点明显高于模型组(P<0.05),组织病理学变化各时间点明显好于模型组,ICAM-1免疫阳性血管数较模型组减少,差异有统计学意义(P<0.05)。结论:1.黄芪注射液可显著改善大鼠脊髓缺血再灌注引起的后肢神经功能障碍和组织病理学改变,表明黄芪注射液对大鼠脊髓缺血再灌注损伤有明显的保护作用。2.黄芪注射液降低大鼠脊髓缺血再灌注后缺血脊髓ICAM-1的表达,可能为黄芪注射液保护脊髓缺血再灌注损伤的机制之一。

Objective：To investigate the effect of Huangqi injection on spinal cord ischemia-reperfusion injury and explore its related mechanisms. Methods：The rat model of spinal cord ischemia-reperfusion injury was established by abdominal arota block and reperfusion 30 minutes after the block. The rats in Huangqi group were preconditioned with Huangqi injection at the dose of 12 ml/kg 30 minutes before the abdominal arota block. Neural function score was made and the pathological changes of the spinal cord were observed after the surgery. Meanwhile, immunohistochemistry assay was used to measure the expression of intercellular adhesion molecule-1 （ICAM-1）. Results：The neural function scores in Huangqi group at different time points were obviously higher than those in the model group （P〈0.05）, with slighter pathological changes and less ICAM-lpositive vessels in Huangqi group as compared with model group and the differences were statistically signifi- cant （P〈0.05）. Conclusion： 1. Huangqi injection can significantly improve the hind limb dysfunction and histopathological changes resulted from the spinal cord ischemia-reperfusion injury, which suggests that Huangqi injection has a remarkably protective effect on the rat spinal cord ischemia-reperfusion injury. 2. Huangqi injection decreased ICAM-1 expression of the ischemia spinal cord, which might be one of the mechanisms for its protection from ischemia-reperfusion injury.