淋巴瘤组织中疱疹病毒6型特异DNA序列的PCR检测

Detection of type 6 human herpesvirus(HHV 6) specific DNA sequence in lymphoma tissues by PCR

目的探讨人疱疹病毒６型（ＨＨＶ６）与我国淋巴瘤的相关性。方法采用聚合酶链式反应（ＰＣＲ）的方法，检测石蜡包埋的淋巴瘤组织、白血病细胞株及健康献血员外周血淋巴细胞中ＨＨＶ６特异ＤＮＡ序列。结果５２例淋巴瘤组织中，２１例ＰＣＲ扩增出ＨＨＶ６特异ＤＮＡ序列，阳性率４０．４％；４３例献血员外周血淋巴细胞中，８例检测出ＨＨＶ６ＤＮＡ序列，阳性率１８．６％。两者相比，差异有显著性（Ｐ＜０．０５）。两株白血病细胞株也扩增出ＨＨＶ６ＤＮＡ序列。结论ＨＨＶ６与我国淋巴瘤有一定关系。

Objective To study the relationship between human herpesvirus 6 (HHV 6) and lymphoma in China. Methods The specimens of paraffin embedded lymphoma tissue, leukemia cell lines and normal blood donor peripheral blood lymphocytes(PBL) were examined for the presence of HHV 6 DNA sequence by polymerase chain reaction (PCR). Results Of the 52 cases lymphoma studied, HHV 6 DNA sequence was detected in 21 cases ; of 43 normal PBL, HHV 6 DNA sequence was detected in 8 cases. The difference was statistically significant ( P <0.05). HHV 6 DNA sequence was also present in two leukemia cell lines. Conclusion The results suggest that HHV 6 may be related to lymphoma in China.