摘要
目的探讨水通道蛋白1、3、8、9在羊水平衡分子机制中的可能作用。方法收集足月选择性剖宫产分娩的羊水过多和正常孕妇的胎膜组织样本各5例,运用RT-PCR方法检测胎膜组织中水通道蛋白-1、3、8、9mRNA的表达。结果水通道蛋白-9mRNA的表达水平在羊水过多组胎膜组织的水平(1.1403±0.0831)显著高于对照组(0.5903±0.1909)(P=0.002);水通道蛋白-1、3和8mRNA在羊水过多组和对照组胎膜的表达差异无统计学意义(P=0.972,0.242,0.608)。结论水通道蛋白-9mRNA在人羊水过多病例的胎膜组织表达显著升高,在维持羊水量及羊水成分的平衡中可能发挥重要作用。
Objective To determine the expression of aquaporin-1,3,8,9 mRNA (AQP-1,3,8, 9) in amniotic membranes in pregnant women with polyhydramnios. Methods Amniotic membranes were collected from women who presented with either polyhydramnios (n= 5)or normal amniotic fluid volume (control, n=5) underwent elective cesarean sections at term. The AQP-1,3,8,9 mRNA expression were detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Results The expression of AQP-9 mRNA on fetal membranes were significantly higher in polyhydramnios groups (1. 1403±0. 0831) than that of control (0. 5903± 0. 1909) (P= 0.002), although the expression of AQP-1, 3 and 8 showed no significant difference between the two groups (P= 0. 972, 0. 242,0. 608, respectively). Conclusions AQP-9 may play an important role in maintaining the volume and the balance of different components of amniotic fluid in polyhydramnios cases.
出处
《中华围产医学杂志》
CAS
2009年第3期197-200,共4页
Chinese Journal of Perinatal Medicine
基金
国家自然科学基金(30471828),教育部留学回国人员科研启动基金(教外司留[2005]383号),广东省科技计划项目基金(2004836001008),2005年广州市卫生局科研基金(28)
关键词
水孔蛋白质类
基因表达
羊水过多
胎膜
Aquaporins
Gene expression
Polyhydramnios
Extraembryonic membranes