一个完整的人/鼠杂种细胞克隆分布板的建立与鉴定

ESTABLISHMENT AND IDENTIFICATION OF A COMPLETE HUMAN/RODENT HYBRID CELL CLONE PANEL

运用Giemsa染色、G-11分化染色与染色体标记同工酶分析相结合的方法,建立了一个完整的人/中国仓鼠杂种细胞克隆分布板,并进行了鉴定。结果表明,该克隆分布板共包含10个细胞株,对人类的24条染色体均可给出不同的特征性信号,并可用于人染色体的基因定位,对部分缺失的染色体可进行精细的基因区域定位。

Combination of chromosome G-banding, Giemsa-11 differential staining andchromosomal marker isozyme analysis is used to establish and identify a completehuman/rodent hybrid cell clone panel. This panel consists of 10 clones, whichcan be used in mapping genes on the 24 different chromosomes. The deleted chro-mosome No. 3 and 7 combined with other deleted chromosomes published earlier,it is possible to map the genes on chromosomes 3, 4, 5, 7 and 8 regionally.