小剂量氮芥对胰腺炎治疗作用及其机制的研究

Experinmental study of low-dose chlormethine on acute severe pancreatitis

目的:探讨氮芥对重症急性胰腺炎的治疗作用及其可能机制。方法:30只雄性SD大鼠随机分成3组;重症急性胰腺炎组(SAP组,n=10),用3.5%牛黄胆酸钠逆行性胆胰管注射后钳夹胆胰管末端制作重症胰腺炎模型;氮芥治疗一组(C1组,n=10),制作重症急性胰腺炎模型前8h,氮芥0.1mg/kg腹腔注射;氮芥治疗二组(C2组,n=10),制作重症急性胰腺炎模型前8h,氮芥0.15mg/kg腹腔注射。各组大鼠6h后处死,取标本并检测血清淀粉酶、血糖、血钙及全血白细胞记数。结果:与重症急性胰腺炎组相比,氮芥治疗一组和氮芥治疗二组胰腺、肝脏、肺脏组织损伤明显改善;血浆Ca2+、血清淀粉酶、血糖浓度及全血白细胞记数等生化指标与SAP组相比有统计学意义。结论:氮芥可能通过诱导胰腺细胞发生凋亡而减轻了重症急性胰腺炎SD大鼠的组织损伤。

Objective： To investigate the therapeutical effects and its possible mechanism of Chlormethine on severe acute pancreatitis in rats . Methods ：Fifty male SD rats were randomly devid into three groups：the severe acute pancreatitis group （SAP, n = 10）, the modle was established in rats by retrograde injection of 3. 5% sodium taurocholate into biliopancreatic duce, followed by duct ligation ;the first group of Chlormethine treatment （C1 ,n= 10）, Chlormethine（0. 1mg/kg）was injected intraperitoneally eight hours earlier prior to retrograde injection of the sodium taurocholate ;the second group of Chlormethine treatment （C2,n= 10） ,Chlormethine （0. 15mg /kg ）was injected intraperitoneally eight hours earlier prior to retrograde injection of the sodium taurocholate . Three group rats were all sacrificed six hours later, the levels of serum amylase , glucose ,serum calcium and the count of white cells were detected. Results ：Compared with the SAP gronp ,the injurement of pancreas ,liver and lung in the C1 group and the C2 group were all significantly ameliorated . The levels of serum amylase ,glucose ,serum calcium and the count of white cells all have the statistical differece . Conclusion： Chlormethine can ameliorate the injurement of severe acute pancreatitis in rats probably through induction of pancreatic acinar cell apoptosis .