C-erbB-2、PCNA表达及HPV感染与涎腺肿瘤关系的研究

The relationship of C-erbB-2、PCNA and infections of HPV in salivary gland tumors

目的:探讨人乳头状瘤病毒HPV感染及原癌基因C-erbB-2和增殖细胞核抗原PCNA在涎腺肿瘤中的异常表达与肿瘤发生的生物学特征及临床病理间关系。方法:(1)应用免疫组化LSAB法及图像分析系统对37例涎腺恶性肿瘤、31例涎腺多形性腺瘤、20例涎腺非肿瘤组织中C-erbB-2及PCNA的表达进行检测,并对阳性细胞进行图像分析定量测定。(2)用聚合酶链反应(PCR)检测涎腺良、恶性肿瘤及非肿瘤组织中HPV16、18型DNA。结果:涎腺恶性肿瘤组HPV16、18型DNA阳性率为27.0%,多形性腺瘤组阳性率为9.7%,而涎腺非肿瘤组织无1例阳性,3者间差异有统计学意义(P<0.05)。多形性腺瘤中C-erbB-2及PCNA阳性率均低于涎腺恶性肿瘤,但仅C-erbB-2两者间差异有统计学意义(P<0.05)。结论:C-erbB-2的过度表达可能在涎腺组织良性病变的恶性转化过程中起关键性的作用。PCNA的异常表达在涎腺良、恶性病变存在一定差别,虽无统计学意义,却能反映其变化特点,有待增加更多病例加以证实。HPV16、18型感染与某些涎腺肿瘤的发生有关,值得进一步深入研究。

Objective： To study the altered expression of oncogene C-erbB-2 , proliferating cell nuclear antigen（PCNA） and infections of HPV in human salivary gland tumors related to the hiopathology and clinical significance. Methods ： Expression of C-erbB-2.PCNA and infections of HPV in salivary gland malignant tumors （37）,Pleomorphie adenomas （31） and non-tumor tissues （20） were detected by immunohistoehemistry （LSAB） and PCR ,The results were analyzed by Image Analysis System. Results ..The positive rate of HPV was 27.0% in salivary gland malignant tumor,the positive rate of HPV was 9. 7% in pleomorphic adenomas,but non-tumor tissues the positive rate of HPV of thenon-tumon was zero. The different percentage among them showed a statistic difference （P〈0. 05）. The positive frequency of C-erbB-2 and PCNA in pleomorphic adenomas was much lower than that in salivary gland malignant tumor . However, only C-erbB-2 expression showed a statistic difference （P〈0. 05） . No association of the expression of PCNA and infection of HPV among them either in malignant was found （P〈0.05） . Conclusion：C-erbB-2 overexpression may involve in malignant transformation of benign lesions of human salivary gland. Immunohistochemical examination of oncogene C- erbB-2 .proliferating cell nuclear antigen（PCNA） could be used for moecular evaluation of benign and malignant tumors. The detection of C-erbB-2 may offer more molecular index for the early diagnosis, clinical therapy of salivary malignant and benign tumors. The results suggested that HPV 16.18 infections are related to salivary gland tumor.