氨基酸模式对体外培养瘤胃主要蛋白质降解菌的影响

Effects of amino acids component patterns on the major protein-degrading bacteria in rumen

本研究以4头装有永久性瘤胃瘘管的徐淮山羊为试验动物,利用体外培养法研究了不同氨基酸模式对瘤胃内主要蛋白质降解菌的影响。研究结果表明不同氨基酸模式对不同菌种的作用有所差异:试验组C的氨基酸组成(Lys16.2%、Met5.4%、Thr12.5%、His5.0%、Arg10.2%、Try4.2%、Leu14.7%、Ile10.6%、Phe10.7%、Val10.5%)对嗜淀粉瘤胃杆菌和栖瘤胃普雷沃氏菌的促进生长效应最高;而试验组B的氨基酸组成(Lys16.0%、Met5.4%、Thr11.9%、His4.5%、Arg10.2%、Try3.5%、Leu15.5%、Ile11.1%、Phe10.5%、Val11.5%)对溶纤维丁酸弧菌的促进生长效应最高。

Four Xuhuai wethers fitted with permanent ruminal cannulas were used in this experiment to determine the effects of different amino acids component patterns on the major ruminal protein-degrading bacteria.The results showed that there was a certain effects of different amino acids component patterns on different rumen bacteria：The maximum stimulatory effects of amino acids component patterns on R.amylophilus and P.ruminicola was group C （Lys 16.2 %, Met 5.4 %,Thr 12.5 %,His 5.0 %,Arg 10.2 %,Try 4.2 %,Leu 14.7 %,Ile 10.6 %,Phe 10.7 %,Val 10.5 %）.However, the stimulatory effects of group B on B.fibrisolvens was supreme,which contained Lys 16.2 %,Met 5.4 %,Thr 11.9 %,His 4.5 %,Arg 10.2 %,Try 3.5 %,Leu 15.5 %,Ile 11.1%,Phe 10.5 %,Val 11.5 %.