大鼠烧伤切痂后大张自体皮移植的胶原代谢

Collagen Metabolism after Skin Auto-Grafting in Burned Mice

为研究大鼠烧伤切痂大张自体皮移植后的胶原代谢,应用 ELISA 法测定移植物Ⅰ、Ⅲ型胶原含量和胶原酶含量。应用氯胺 T 氧化法测定羟脯氨酸含量,应用放射免疫法测定血 PIIINP 量。结果表明:伤后第7d,自体皮贴合良好,伤后第28d 创面完全愈合并部分长毛。伤后第7d Ⅰ型胶原最低,以后逐渐恢复,伤后28d 明显上升。伤后羟脯氨酸、PIIINP、Ⅲ型胶原和胶原酶呈上升趋势,其中 PIIINP 在伤后7d 最早出现明显上升,其余在伤后14d 明显上升,直至伤后28d;同时Ⅲ/Ⅰ比也明显上升。

To elucidate collagen metabolism after escharectomy and skin auto-grafting in full tickness burned mice,dynamic changes of type Ⅰ and type Ⅲ collagen and collagenase in skin autografte which survived and recovered its normal physiology property on the wound 28d after transplantation were measured by ELISA.pⅢNP was determined by RIA.Type Ⅰ collagen declined to the lowest level 7th day after transplantation and then increased gradually to significant high level 28th day after transplantation.All levels of hydroxyproline、PⅢNP、type Ⅲ collagen and collagenase increased significantly 14th day post-auto-graft.The level of PⅢNP increased significantly 7th day post-auto-graft.