甲基丙烯酸环氧丙酯诱导哺乳类细胞转化的实验研究

Study on the Transformation of Mammal CellsInduced by Glycidyl Methacrylate in Vitroc

用人胚肺成纤维细胞的体外转化实验检测出甲基丙烯酸环氧丙酯可诱导人胚肺成纤维细胞形态学改变，转化细胞核浆比例增大，排列紊乱，交叉重叠生长。从转化集落分离的细胞可被较低浓度的刀豆凝集素Ａ凝集，在半固体琼脂内非贴壁依赖生长。转化细胞的染色体发生结构和数目改变。上述结果表明：甲基丙烯酸环氧丙酯具有诱导人胚肺成纤维细胞恶性转化的作用，预示它可能对人具有潜在的致癌危险性。

lycidyl methacrylate(GMA) can induce the phenotype transformation of human embryonic lung fibroblasts.The transformed cells prolifeated rapidly with a higher in the ratio of nucleus to cytoplasm,and their life span extended notably.The transformed colonies exhibited in extensive random orientation and the cells crossing over.The transformed cells could be agglutinated by lower concentration of ConA and could grow in semisolid agar.The chromosome abberations could occur in transformed fibroblasts.These results suggest that GMA is able to induce malignant transformation of mammal cells,and it may be carcinogenic to human.