自身免疫性甲状腺疾病免疫学检查的临床评价

CLINICAL EVALUATION OF SPECIFIC IMMUNOLOGICAL ASSAYS IN AUTOIMMUNE THYROID DISEASES

在纯化甲状腺球蛋白(TG)和甲状腺微粒体(TM)抗原的基础上,建立了测定抗TG、扰TM,IgG、IgA、IgM类抗TG、抗TM以及TG含量、TG-抗TG循环免疫复合物(TG-IC)的ELISA试验。此组免疫学检查方法具有较高的特异性和敏感性,用于桥本甲状腺炎(HT)、甲状腺机能亢进、甲状腺机能减低等自身免疫性甲状腺疾病(AITD)的临床研究,有助于提高诊断水平和探讨发病机理。

After purifying thyroglobulin (TG) and thyromicrosomal (TM) antigens,the ELISA (enzyme-linked immunosorbent assay) for detecting anti-TG,anti-TM,serum TG level,TG-antithyroglobulin immune complex (TG-IC) and IgG-,IgM-,IgA-specfic antithyroglobulin,antithyromicrosome were established.These specific immunplogical assays were sensitive,reproducible and did not have the shortcomings associated with the impurity of the TM antigen.The results of clinical studies in patients with Hashimoto's thyroiditis (n=35),hyperthyroidism (n=86),primary hypothyroidism (n=7) suggested that these specific assays were helpful to improve the diagnosis and investigation into pathogenesis for aformetioned autoimmune thyroid diseases (AITD).