血管紧张素转化酶基因多态性与高血压病合并脑梗塞的探讨

THE RELATIONSHIP BETWEEN ANGIOTENSIN CONVERTING ENZYME GENE POLYMORPHISM AND BRAIN INFARCTION IN CHINESE HYPERTENSIVES

目的探讨血管紧张素转化酶（ＡＣＥ）基因插入／缺失（Ｉ／Ｄ）多态性与中国人高血压病合并脑梗塞的关系。方法应用聚合酶链反应（ＰＣＲ）方法检测６２例正常人，５５例高血压病无心脑血管合并症患者，４４例高血压病合并脑梗塞患者的ＡＣＥ基因型。结果ＡＣＥ基因Ｉ／Ｄ多态性与高血压病无相关关系。但高血压合并脑梗塞的ＤＤ基因型频率２９．６％及Ｄ等位基因频率５６．８％显著高于正常对照组的１２．９％和３８．７％（分别Ｐ＜０．０５和Ｐ＜０．０１），以及高血压无心脑血管合并症组的１２．８％和４０％（分别Ｐ＜０．０５，Ｐ＜０．０２）。

Objective To identify the insertion/deletion(I/D) polymorphism of angiotensin converting enzyme(ACE) gene in Chinese hypertensives complicated with brain infarction. Methods ACE genotyping with polymerase chain reaction(PCR) was performed in 62 unrelated healthy controls, 55 hypertensives without cardio cerebrovascular diseases and 44 hypertensives complicated with brain infarction(all controls had no hypertension family history, while the cases had hypertension family history). Results No significant differences could be detected between ACE gene I/D polymorphism and hypertension. However, the frequencies of DD genotype and deletion allele among hypertensives complicated with brain infarction(29.6% vs 56.8%) were higher than those among healthy controls (12 9% vs 38.7%, P <0 05 and P <0 01) and those among hypertensives without cardio cerebrovascular diseases (12.8% vs 40%, P <0.05 and P <0 02). Conclusion A deletion polymorphism of ACE gene is probably an important hereditary factor of brain infarction's morbidity in Chinese hypertensives. The detection of ACE genotypes in hypertensives would improve the early diagnosis of brain infarction.