七氟醚预处理对大鼠内毒素性急性肺损伤的影响

Effects of sevoflurane preconditioning on LPS-induced acute lung injury in rats

目的探讨七氟醚预处理对大鼠内毒素性急性肺损伤的影响。方法健康雄性SD大鼠24只，体重220—250g，随机分为4组（n=6）：对照组（C组）、内毒素组（LPS组）、七氟醚组（Sev组）和七氟醚预处理组（SP组）。C组和LPS组机械通气30min后分别静脉注射生理盐水和脂多糖（LPS）5Sag／kg；Sev组和SP组吸入七氟醚．（呼气末浓度2．4％）30min，洗脱5min，然后分别静脉注射生理盐水和LPS5mg／kg。于给予LPS或生理盐水后6h时，心脏放血处死大鼠，取肺组织，计算湿重，干重（W／D）比；采用弥漫性肺泡损伤（DAD）评分评价肺组织损伤程度；测定肺组织髓过氧化物酶（MP0）活性、细胞因子诱导中性粒细胞化学趋化因子-1（CINC-1）含量、CINC-1和CINC-1mRNA的表达水平。结果与C组比较，LPS组和SP组肺组织W／D比、DAD评分、MPO活性和CINC-1含量升高，CINC-1和CINC-1 mRNA的表达上调（P〈0．01），Sev组上述指标差异无统计学意义（P〉0．05）；与LPS组比较，SP组肺组织W／D比、DAD评分、MPO活性和CINC-1含量降低，CINC-1和CINC-1 mRNA的表达下凋（P〈0．01）。结论七氟醚预处理可减轻大鼠内毒素性急性肺损伤，可能与其抑制肺组织炎性反应有关。

Objective To investigate the effects of sevoflurane preconditioning （SP） on acute lung injury induced by lipopolysaecharlde （LPS） in rats. Methods Twenty-four male SD rats weighing 220-250 g were randomly divided into 4 groups （ n = 6 each） ： group Ⅰ control （group C）, group Ⅱ LPS, group Ⅲ sevoflurane （group Sev） and group Ⅳ SP ＋ LPS. In group Ⅰ and Ⅱ , normal saline and LPS 5 mg/kg were given Ⅳ 30 min after ventilation respectively. In group Ⅲ and Ⅳ, the animals inhaled sevoflurane （end-tidal concentration 2.4% ） for 30 min followed by 5 min wash-out, and then received iv injection of normal saline and LPS 5 mg/kg respectively. The animals were killed at 6 h after LPS or normal saline administration. Lungs were removed for determination of W/D lung weight ratio, myeloperexidase （MPO） activity, cytokine-induced neutrophil chemoanractant-1 （CINC-1） content and expression of CINC-1 and CINC-1 mRNA. The severity of lung injury was evaluated using diffuse alveolar damage （DAD） score. Results Compared with group I , W/D lung weight ratio, DAD score, MPO activity and CINC-1 content were significantly increased, and expression of CINC-1 and CINC-1 mRNA up-regulated in group Ⅱ and Ⅲ, while there was no significant difference in the above indices between group Ⅲ and group Ⅰ . Compared with group Ⅱ , W/D lung weight ratio, DAD score, MPO activity and CINC-1 content were significantly decreased, and expression of CINC-1 and CINC-1 mRNA down-regulated in group Ⅲ . Conclusion Sevoflurane preconditioning can protect the lungs against LPS-induced acute lung injury by inhibiting inflammatory response.