苦豆子内生放线菌的分离鉴定及其拮抗菌筛选

Identification of Endophytic Actinomycetes and Screen of Antagonist from Sophora alopecuroides L.

采用3种消毒时间对健康苦豆子植株体内的内生放线菌进行分离。结果表明:消毒3 min消除非内生菌的影响效果较好;同一植株分离内生放线菌的数量,根部比茎、叶部多。经初步鉴定,苦豆子内生放线菌以链霉菌属和诺卡氏菌属为最多,分别占分离总数的35%和27%;其次为小多孢菌属,占12%;而类诺卡氏菌属、小单孢菌属、孢囊放线菌属和分枝杆菌属分离较少。26株纯化菌株对茄子枯萎病菌进行平板对峙培养,筛选出3株抑菌带宽度达到10 mm以上的菌株,其中菌株KDS22发酵液抑菌效果最好。

Endophytic actinomycetes were isolated from healthy Sophora alopecuroides L. with 3 sterilizing time. The results indicated that sterilized disposal in the 3 rain were the optimal methods of wiping off nor endophytes endophytes. Quantities of endophytic actinomycetes from different parts of Sopkora alopecuroides L. varied and there were more endophytic actinomycetes in the roots than in the stems and leafs for the same plant. It was showed on primary identification that Streptomyces and Nocardia were more than ones of that endophytic actinomycetes from Sophora alopecuroides L., 35% and 27% in total isolations respectively. Micropolyspora was 12%, however, Nocardioides, Micromonospora, Actinosporangium and Mycobacterium were less in isolations. The 26 purified endophytic actinomycetes were cultured confronting to eggplant fusarium wilt to screen high resistant strains. 3 strains with 10 mm inhibition zone more were obtained. Of which the free-cell fermentation of KDSz2 strain showed higher resistant to pathogen than other ones.