中国特有种虎榛SSR反应体系的初步研究

DNA Extraction and Construction on the SSR Reaction System of Ostryopsis Davidiana Decne

为了探讨适于中国特有种虎榛DNA提取的方法和建立虎榛的SSR反应体系,该试验以虎榛幼嫩叶片为材料,通过对CTAB法的改良,摸索出了适于虎榛DNA的提取方法,并以核酸产量、纯度、片断分布情况等指标进行了评价,同时应用16对欧榛SSR引物对虎榛进行了跨属转移,获得了清晰稳定的扩增图谱;上述结果表明获得的基因组质量较高,同时也表明反映体系对虎榛的SSR分析是可行的;并对影响虎榛基因组DNA的制备及扩增反应因素进行了简要的分析讨论。

This study presented to probe DNA extraction and to construct the SSR reaction system of Ostryopsis davidiana Decne. A procedure for DNA extraction from the leaves of Ostryopsis davidiana Decne was constructed by modifying the method CTAB. Quality of DNA was evaluated by yield and purity of genomic DNA and gel eletrophoresis inspection, at the same time, the genome DNA of Ostryopsis davidiana Decne was amplified using 16 pairs of SSR corylus primers and obtained clear and stable amplification patterns, which indicated the DNA was good and the system was feasible. The factors affecting the DNA extraction and amplification of genome DNA of Ostryopsis davidiana Decne were discussed in the paper.