花椰菜雄性不育系组培快繁及无糖培养技术

Rapid Propagation and Sugar-free Tissue Culture for Male-sterile Line Cauliflower

以花椰菜雄性不育亲本的花球为外植体,进行组培快繁及无糖生产技术的研究。结果表明:在MS+BA 1.0 mg/L+NAA 0.3 mg/L的培养基上诱导,25 d时不定芽的分化率达到96%,继代增殖在MS+BA 0.6 mg/L+NAA 0.1 mg/L的培养基效果最好,增殖率可达4.25倍。无糖组织培养的培养基为MS无机成分+NAA 0.1,光照4 000 lx,CO2浓度1 000 mL/L,11 d可大量生根。通过上述方法,1个花球,在4个月可生产上万株组培苗,是一种高效快速的方法。

Useci flower ball of Male-sterile line cauliflower as the explants for tissue culture and sugar-free technological research. The results showed that..when Induced 25 d differentiation rate of adventitious bud in the MS＋BA1.0 mg/L＋ NAA 0. 3 mg/L was 96%. The best medium of subculture was MS＋BA 0. 6 mg/L＋NAA 0. 1 mg/L. The proliferation rate was 4. 25 times. The medium of sugar-free tissue culture was MS inorganic component ＋NAA 0. 1. At light 4 000 lx and CO2 concentrations 1 000 mL/L, 11 d could be substantial root. Through this method, a flower ball, in the 4 months can produce more than 10 000 plantlets, this was a highly effective method.