仙客来愈伤诱导抗褐化研究

Cyclamen Callus Induction Research on Anti-browning

以仙客来(Cyclamen persicum Mill)法国莫莱尓F2代植株为材料,选取同一植株的叶片,叶柄和块茎做为外植体进行愈伤诱导,选取最佳愈伤诱导培养基,针对愈伤诱导过程中出现的褐化问题,从不同方面入手,寻求最佳的褐化控制培养基和培养条件,解决组织培养中褐化问题。结果表明:最佳愈伤诱导培养基是MS+6-BA(0.5 mg/L)+2,4-D(2 mg/L)+KT(0.2mg/L)+琼脂(9 g/L)+蔗糖(30 g/L);最佳褐化控制培养基是在愈伤诱导培养基中添加Vc(2mg/L)或PVP(0.5 mg/L);暗培养能一定程度上抑制褐化的发生;活性炭虽能抑制褐化但同时也抑制仙客来愈伤组织的生长。

The experimental used Cyclamen （Cyclamen persicum Mill） France Murray Mi F2 plants as the materials, selected the same plant leaf, petiole, and tubers as explants to callus induction,to chose the best medium callus. Callus for induction in the process of browning were discussed from different aspects, found the best browning control of the medium and culture conditions, results showed that： the best callus medium was MS＋ 6-BA（0. 5 mg/L）＋2, 4-D （2 rag/L）＋KT（0. 2 nag/L）＋agar（9 g/L）＋sugar（30 g/L） ;best browning control training callus-induction in the medium to add Ve（2 mg/L）or PVP（0. 5 mg/L）;dark to a certain extent, curb the occurrence of browning; Although the activated carbon could inhibit browning but also inhibit Cyclamen callus growth.