香菇液体培养的工艺研究

Technical Research on the Liquid Cultivation of Mushroom

运用单因素对比试验和正交试验,对香菇液体培养基的碳、氮营养源以及最佳配方和培养条件进行了优化研究,筛选出最佳碳源为麦芽糖,最佳氮源为麸皮,确定最佳培养基配方为麸皮80%、麦芽糖3%、MgSO40.15%、KH2PO40.1%;最佳培养条件为pH值5.5,摇瓶装液量100 mL/250 mL,接种量15%,摇瓶转速160 r/min。该研究为香菇液体发酵生产提供借鉴和理论依据。

Through experiment of single element contrast and orthogonal experiment, an optimized research was carried out on the resource of carbon and nitrogen, the best formula and cultivating condition of liquid culture medium of mushroom. Malt sugar was decided as the best carbon resource, bran as the best nitrogen resource. The best formula of culture medium was 80% bran, 3% malt sugar, 0. 15% MgSO4 and 0. 1% KH2PO4. The best cultivating condition was pH value 5.5, shake flask content 100 mL/250 mL, inoculum concentration 15%, shake flask speed 160 r/min. The research provided mushroom liquid fermentation production with a theoretical basis and guidance.