不同种源益母草遗传关系的ISSR分析

Genetic relationship analysis of different provenances of Leonurus japonicus by ISSR marker

目的:阐明不同种源益母草的遗传关系。方法:用ISSR分子标记研究来自益母草主要分布区19个种源样本。结果:从100个ISSR引物中筛选出20个条带清晰、多态性高的引物,共扩增出164条带,其中117条带为多态性位点,多态性条带比率(PPB)为71.34%,通过聚类分析,可将19个益母草种源聚为3类。结论:分子标记研究结果与益母草的地理位置、形态性状存在显著的相关性,较好地揭示了益母草种源间的遗传关系,可为益母草资源划分和良种选育提供科学依据。

Objective： To study the genetic polymorphism and genetic relationship of different provenances of Leonurus japonicus. Method： Genetic relationship in 19 provenances, which came from nationwide origins were measured by using polymerase chain reaction （PCR） with ISSR marker. Result： Twenty ISSR primers were selected from 100 ISSR primers and used for ISSR amplification. A total of 164 bands were generated, of which 117 bands were polymorphic bands （the percentage of polymorphic band, PPB = 71.34% ）. The similarity coefficients were calculated with NTsys 2.10e software and the dendrogram were constructed with UPGMA. Nineteen samples were divided into 3 types using the cluster analysis according to their genetic similarity coefficient. Conclusion： Results from the cluster analysis were correlated significantly with the morphological characteristics and geographical location of 19 sampies. The data indicate that ISSR technique is useful to determine genetic diversity and genetic relationship among Leonurus provenances, providing a scientific basis for genetic breeding, differentiation and new cultivar selection.