短柄小连翘中的木脂素类化学成分研究

Ligans from branch of Hypericum petiolulatum

目的:对短柄小连翘Hypericum petiolulatum的化学成分进行系统的研究。方法:采用硅胶、大孔吸附树脂、Sepha-dex LH-20柱色谱和制备型高效液相色谱方法进行分离;应用NMR和MS等波谱学方法鉴定化合物的结构。结果:从短柄小连翘乙醇提取物中分离得到9个木脂素类成分,分别鉴定为(-)-(2R,3R)-1-O-feruloyl-8,8′-bisdihydrosiringenin(1),(-)-secoisolariciresinol 4-O-β-D-glucopyranoside(2),isolaricire-sinol-β-4′-O-β-D-glucopy-ranoside(3),5-methoxy-9β-xylopyranosyl-(+)-isolariciresinol(4),(+)-lyoniresinol-2α-O-β-D-xylopyranoside(5),5-methoxy-9-β-xylopyranosyl-(-)-isolariciresinol(6),isolaricir-esinol 6a-O-β-D-glucoside(7),(+)-lyoniresinol 3α-O-β-D-xylopyranoside(8)和7-methoxy-5-benzofuranpropanol 4′-O-β-D-glucopyr-anoside(9)。结论:化合物1为一新化合物,其余化合物均为首次从该属植物中分离得到。

Objective： To investigate the chemical constituents of Hypericum petiolulatum. Method ： The chemical constituents were isolated and purified by column chromatography on silica gel, macroporous adsorbent resin, Sephadcx LH-20, and preparative HPLC. Structures of the compounds were identified by NMR and MS spectroscopic methods. Result： Nine lignans were obtained and their structures were elucidated as （ - ）-（ 2R, 3R）-1-O-feruloyl-8,8＇-bisdihydrosiringenin （1）, （ - ）-secoisolariciresinol 4-O-β-D- glucopyrano-side （ 2 ）, isolariciresinol-β-4＇-O-β-D-glucopyranoside （ 3 ）, 5-methoxy-9β-xylopyra-nosyl-（ ＋ ） -isolariciresinol （ 4 ） , （ ＋ ） -lyoniresinol 2α-O-β-D-xylopyranoside （ 5 ）, 5-methoxy-9-β-xylopyranosyl-（ - ） -isolariciresinol （ 6 ） , isolariciresinol 6a-O-β-D- gluco-side （ 7 ）, （ ＋ ） -lyoniresinol 3α-O-β-D-xylopyranoside （ 8 ） and 7-methoxy-5-benzofuranpropanol 4＇-O-β-D-glueopyranoside （9）. Conclusion： Coupound 1 was new and compounds 2-9 were obtained from the genus Hypericum for the first time.