假奓包叶抗菌活性成分的研究(2)

Studies on antibacterial constituents of Discocleidion rufescens(2)

目的：研究假奓包叶的抗菌活性成分。方法：在抗菌药理活性跟踪下，应用多种色谱技术进行化合物分离，运用波谱分析法和化学手段鉴定其结构。结果：从乙醚萃取部位和正丁醇萃取部位共分离得到7个化合物，分别鉴定为5，7-二羟基4’-甲氧基黄酮（1）、槲皮素（2）、洋芹素-7—O-β-D-葡萄糖苷（3）、洋芹素-7-O-新橙皮苷（4）、木犀草素-7-O-新橙皮苷（5）、没食子酸（6）和β-胡萝卜苷（7）。结论：化合物1—6为首次从假奓包叶属植物中得到，并有一定的抑制大肠杆菌的活性；化合物6还具有抑制金黄色葡萄球菌的活性。

Objective： To screen effective constituents of Discocleidion rufescens against bacteria. Method ： Compounds were isolated by bioassay-guided fractionation method with various chromatographic methods and their structures were determined by spectral analysis and chemical evidence. Result： Seven compounds were isolated from the Et20 and n-BuOH extracts and identified as 5,7-dihydroxy-4 ＇-methoxy flavone （ 1 ） , quercetin （ 2 ）, apigenin-7-O-β-D-glucopyranoside （ 3 ）, apigenin-7-O-neohesperidoside （ 4 ） , luteolin-7-O-neohesperidoside （5）, gallic acid （6） andβ-daucosteol （7）. Conclusion： Compounds 1-6 were isolated from genus Discocleidion for the first time and exhibited certain inhibitory activity against Escherichia coli. Compound 6 also showed antibacterial activity against Staphylococuus aureus.