川芎防风白芷方主成分及其组合对家兔离体脑基底动脉的作用

Effect of Chuanxiong Fangfeng Baizhi prescription on isolated rabbit basilar artery

目的:观察川芎防风白芷方主成分及其组合对家兔离体脑基底动脉环的作用。方法:分别以氯化钾(KCl)45mmol.L-1和5-羟色胺(5-HT)2.4×10-8mol.L-1预收缩家兔离体脑基底动脉环,采用累积加药的方式,观察川芎防风白芷方主成分及其组合(P1,P2)对预收缩血管环的舒张作用。结果:KCl 45 mmol.L-1预收缩时,除防风色原酮Ⅰ葡萄糖苷、防风色原酮Ⅱ葡萄糖苷无明显舒张作用外,藁本内酯、阿魏酸、欧前胡素、P1及P2舒张血管的作用显著,最大舒张率可接近100%,(EC50):P2<欧前胡素<藁本内酯<P1(P<0.05)。5-HT2.4×10-8mol.L-1预收缩时,2个主成分组合样品均能产生明显的舒张作用,并呈剂量-反应关系。结论:除防风色原酮Ⅰ葡萄糖苷、防风色原酮Ⅱ葡萄糖苷外,其他单体成分及主成分组合样品均能对抗KCl及5-HT引起的家兔基底动脉收缩,且主成分组合样品的舒张作用优于单体成分。

Objective： To observe the effect of the main constituents and its combination from Chuanxiong Fangfeng Baizhi prescription on isolated rabbit basilar artery. Method： The isolated rabbit basilar artery rings were treated with 5 mmol · L^- 1 KCl and 5- HT 2.4×10^-8 mol ·L^ -1, and by measuring the tension of basilar artery rings, the effect of the main constituents and its combination from Chunxiong Fangfeng Baizhi Prescription on vascular activity was observed. Result： Under pre-contractions of KCl45 mmol·L^-1 , ligustilide, ferulic acid, imperatorin and two constituentg combination have the vasodilatation effect on rabbit basilar artery rings white prim-o-glucosylcimifugin and 4＇-O-β-D-glucosyl-5-o-methylvisamminol have no same action. The EC50 sequence is P2 〈 imperatorin 〈 ligustilide 〈 P1 （ P 〈 0. 05 ）. And the same vasodilatation effect on rabbit isolated basilar artery rings evoked by 5-HT 2.4×10^-8 mol·L^-1 was found for two constituents＇ combination. Conclusion： Except prim-o-glucosyleimifugin and 4＇-O-β-D-glucosyl-5-o-methyl- visamminol, all samples have the vasodilatation effect on rabbit isolated basilar artery rings evoked by KCI and 5-HT, the vasodilatation effect of constituent＇s combination were better than any single constituent.