醋酸水解三七总皂苷制备人参皂苷Rg3工艺的优选

Process optimization of ginsenoside Rg3 prepared by hydrolysing Panax Notoginseng saponins by acetic acid

目的:寻找醋酸水解三七总皂苷制备人参皂苷Rg3(Ginsenoside Rg3)的最佳工艺。方法:考察醋酸体积分数、水解温度、皂苷体积分数、水解时间4个因素对人参皂苷Rg3产量的影响;在单因素试验的基础上进行L9(34)正交试验,以总水解产物[主要含20(R)-人参Rg3]的质量为考察指标。结果:醋酸水解三七总皂苷制备人参皂苷Rg3的最佳工艺条件为:醋酸体积分数30%、水解温度90℃、皂苷体积分数5%、水解时间1h;本试验所确定的最佳制备工艺制备人参皂苷Rg3的得率为30%,HPLC法检测总转化产物中20(R)-人参皂苷Rg3含量达80%以上,旋光分析其光学纯度为78.33%。结论:该法是一种省时、省力、简便、快速制备较高纯度的20(R)-Rg3的可行的方法。

OBJECTIVE To find the best process of ginsenoside Rg3 preparated by acetic acid hydrolysing panax notoginseng saponins. METHODS The influencing factors were investigated： acetic acid concentration, temperature, saponin concentration and time; basing on the single factor experiment, the L9 （3^4 ）orthogonal test was prepared. The quality of ginsenoside Rg3 from hydrolysate was used as index,and the experiment was repeated three times. RESULTS The best process was as flows： the acetic acid concentration was 30%, the temperature was 90 ℃, the saponin concentration was 5%o and the time is three hours; the yield of 20（R）-ginsenoside Rg3 prepared by this process was 30% calculated by the conversion of panaxadiol sapo nins. and optical purity was 78.33%. CONCLUSION The method is easy to operate and is a feasible method to prepare high purity 20（R）-ginsenoside Rg3.