ADAM33基因单核苷酸多态性与成人哮喘病情分级的关系

ADAM33 polymorphisms with asthma susceptibility and severity in adolescent asthma

目的探讨ADAM33基因他、S2位点单核苷酸多态性对成人哮喘的遗传易感性与病情严重程度的相关性。方法选取他、S2位点，对186名哮喘患者（病例组）和150名正常者（对照组）采用直接测序方法进行研究。结果病例组与对照组，T2、S2位点的基因型构成比较差异有统计学意义（T2：Χ^2=4．97，P=0．026；S2：Χ^2=7．729，P=0．021）。两位点均能增加哮喘发生的相对危险度（T2：P=0．027；S2：P=0．024），但在成人哮喘的病情严重程度上基因型构成差异比较无统计学意义（T2：Χ^2=-0．13，P=0．897；S2：Χ^2=0．549，P=0．760）。结论ADAM33基因，T2、S2位点单核苷酸多态性与成人哮喘易感性相关，增加哮喘危险度，但与成人哮喘病情严重程度无显著相关性。

Objective To verify ADAM33 （T2, S2） polymorphisms with asthma susceptibility and severity in adolescent asthma. Methods According to the LD pattern, we selected 2 SNPs （ T2, S2） and searched for single nueleotide polymorphisms（SNPs） in ADAM33 by PCR-direct sequencing for 186 adult asthmatic patients and 150 normal controls. Results There was a significant difference in the distribution of the genotypes of the two SNPs between adolescent asthmatics and normal controls （P = 0. 026 and 0.021 ,respectively）,and both of the SNPs could increase the relative risk （T2： P = 0. 027;S2： P = 0.024）. No significant difference was found with adolescent asthma severity（ T2：Χ^2 = -0.13,P = 0.897; S2 ： Χ^2 = 0. 549, P = 0.760）. Conclusion Single nucleotide polymorphism of ADAM33 was revelant with adolescent asthma susceptibility, which can increase the relative risk, but there is no relation with adolescent asthma severity, maybe it can make progress in the childhood.