丁型肝炎病毒和乙型肝炎病毒感染关系的探讨

STUDY ON THE RELATIONSHIP OF HDV AND HBV INFECTION

用免疫组化法检查283例肝组织切片的HDV染色,检出HDV39例(13.8％)。其中1例未查HBVM;19例HBVM阴性;263例曾有HBV感染的肝组织内检出HDV者39例,占14.8％(199例HBsAg阳性病例中肝组织内检出HDV者36例,占18.1％,64例HBsAg阴性者检出3例,占4.7％)。国内HDV感染分布不一,除可能与地区、调查样本大小有关外,特别是与调查对象HBV感染的临床诊断分型更有密切关系。慢性乙肝HDV检出率明显高于急性乙肝,提示HDV感染可能是HBV感染慢性化因素之一。同时,大多数(87.2％)情况下,肝组织内可同时检出HDV和HBsAg、HBcAg,单独检出HDV者是少数(12.8％),说明HDV和HBV间存在密切关系。

HDV was demonstrated in the hepatocytes of 39 samples (13.8%) out of 263 liver tissue sections biopsied from AH,CPH,LC and PHG patients by immuno-histological stain.HDV was not found in 19 samples with negative HBVM and 1 sample in which HBVM was not tested.So,all 39 sampled (14.8%) with positive HDV belonged to the 263 patients with positive HBVM.Among the 199 cases with positive HBsAg in blood,HDV was found in liver tissue of 36 patients (18.1%),but in 64 cases with negative HBsAg,HDV was demonstrated only in that of 3 patents (4.7%)..The distribution of HDV infection was not even in China according to the published literature.This perhaps was related to the sample size of investigation and,especially,the clinical diagnosis of the HBV infection of the investigated patients.Our investigation has proved that the detectable rate of HDV infection in chronic HBV infection was significantly higher than in acute infection,indicating that HDV infection might be a cause of chronicitization of HBV infection.The fact that HDV was accompanied with HBsAg and/or HBcAg in 87.2% liver tissue sections,suggested there was a relationship between HDV and HBV infection.