环氧化酶2在大肠癌中的表达及其与大肠癌生物学特性的关系

Study on the expressions of cyclooxygenase-2 in human colorectal carcinoma

[目的]通过观察环氧化酶2(Cyclooxygenase-2,COX-2)在大肠腺瘤、大肠癌中的表达及其与大肠癌生物学特性的关系,初步探讨其在大肠癌发生、发展过程中的作用机制。[方法]应用免疫组织化学染色法对78例大肠癌组织、21例大肠腺瘤组织和13例正常大肠黏膜组织进行免疫组化染色;应用χ2检验分析COX-2的表达情况及其与大肠癌临床病理特征的关系。[结果]大肠癌组织中COX-2阳性表达率为78.21%,明显高于大肠腺瘤的52.38%和正常大肠黏膜组织的7.69%(P<0.05);COX-2表达与大肠癌的Duke’s分期、分化程度、淋巴结转移有相关性(P<0.05),而与大肠癌患者的性别、组织学类型无关(P>0.05)。[结论]COX-2在大肠癌组织中表达率明显高于大肠腺瘤,而正常大肠黏膜中表达率极低或不表达;COX-2表达与大肠癌生物学特性有明显相关性。

[Objective]To investigate the mechanism of COX-2 in the origin and development of colorectal carcinoma by observing the expressions of COX-2 as well as its correlations with the bionomics of colorectal carcinoma. [Methods]The expressions of COX-2 were determined by immu- nohistochemical staining in 78 colorectal carcinoma , 21 colorectal adenomas and 13 normal mucosal tissues. The expressions of COX-2 in carcinoma, adenoma, and normal tissue were analyzed statistically by X^2 test. [Results]The positive rate of COX-2 in colorectal carcinoma was 78. 21%, which was markedly higher than that in colorectal adenomas （52. 38%） and normal mucosal tissues （7. 69 %） （P〈0. 05）. The expression of COX-2 was significantly correlated with lymph node metastasis, DukCs stage, differentiation of tumor （P〈0. 05）. No significant correlation was noted between COX-2 expression and sex, histologic type（P〉0. 05）. [Conclusion]The positive rates of COX-2 in colorectal carcinoma was obviously higher than that in colorectal adenoma and in normal tissue. The expression of COX-2 was significantly correlated with the clinicalpathology of colorectat cancer.