黄芩苷锌对PC_(12)细胞缺氧缺糖损伤的保护作用

Investigation on the Neroprotective Effects of the HBZn

目的:检验黄芩苷锌(HBZn)是否对神经细胞具有保护功能,并初步探讨其保护机制。方法:以缺氧缺糖(oxygen and glucose deprivation,OGD)诱导PC12细胞损伤模型拟脑缺血病理的改变,采用MTT法检测细胞存活率,研究黄芩苷锌的体外神经细胞保护活性。结果:黄芩苷锌对PC12细胞OGD损伤的保护作用与黄芩苷、灯盏花素的保护效果接近,但在OGD损伤前2h给药保护作用明显。结论:黄芩苷锌对OGD诱导PC12细胞的损伤有预防保护作用。

Objective ： To search for the neuroprotective of HBZn and investigate its mechanisms of neuroprotection. Method： Pheochromocytoma cell （PC12） injury model were induced by oxygen and glucose deprivation （OGD） to simulate the cerebral Ischemia -like changes. MTr were used to test the cell survival rate to observe the neuron - protective activity of HBZn. Results ： The neuron - protective activity of HBZn was similar to that of Baicalin and Breviscapinun, but when HBZn, Baicalin and Breviscapinun were added 2 hours before OGD, their neuron -protective activity was significantly strong, especially HBZn. Conclusions： HBZn possesses neuron -proteetive activties against the OGD -induced PC12 cell injury.