Siglec-1与原发性胆汁性肝硬化的相关性研究

Studies on Correlation of Siglec-land Primary Biliary Cirrhosis

目的检测Siglee-1（sialic acid—binding immunoglobulin—like lectins，唾液酸结合的免疫球蛋白样凝集素，CD169）在原发性胆汁性肝硬化（primary biliary cirrhosis，PBC）患者外周血单核细胞上的蛋白及mRNA表达水平，并探讨其在原发性胆汁性肝硬化发生发展中的作用。方法流式细胞术检测45例PBC患者及36例健康对照者、40例肝炎后肝硬化对照者外周血CD14，CD169双阳性细胞的表达率；实时荧光相对定量RT—PCR方法检测入选对象单核细胞中Siglec—1mRNA的含量；生化常规测定所有入选者血清生化指标水平。结果流式细胞术检测结果显示：PBC组单核细胞cD14，CD169双阳性率为13．0％±2．5％，显著高于健康对照组（1．0％±0．2％，P〈0．01）及肝炎后肝硬化对照组（4．1％±0．5％，P〈0．01）。PBC组Siglec—1mRNA的相对表达量为健康对照组的3．42倍，差异具有统计学意义（P〈0．01）。结论PBC患者单核细胞表面siglec-1蛋白表达显著增高，mRNA含量显著增加，说明PBC患者外周血单核细胞已经发生巨噬细胞化，单核巨噬细胞介导的免疫炎症反应在PBC发生发展过程中起重要作用。

Objective To study on correlation of expression of Siglec-land the course of primary biliary cirrhosis （PBC）. MethodsPeripheral blood from 45 patients with PBC, 36 healthy controls and 40 disease （liver cirrhosis after HBV infection）controls were collected and the positive ratio of CD14＋CD169＋ cells were assayed by flow cytometry（FCM）. Total RNA was extracted from monocytes of these samples. Real time Fluorescence Quantitative Polymerase Chain Reaction （RFQ-PCR）was performed to assay the level of Siglec-lmRNA. The levels of GGT and ALP in sera were evaluated by routine tests. Results It was showed that CD14＋CD169＋ cells increased significantly compared with normal controls and disease controls （13.0 ±2.2 ）% versus （1.0 ±0. 2）%, （4. 1±0. 4） %, P 〈 0. 01. Consistantly, expression of Siglec- 1mRNA increased markedly（relative expression as 3.42 versus 1 as normal controls,P〈0.01）. In addition,expression of Siglec-1protein was related to the levels of GGT（r=0.44,P〈0. 01）and ALP（r=0. 32,P〈0. 05）. Conclusion Inflammation mediated by mononuclear macrophage play important roles in the course of PBC.