耐甲氧西林金黄色葡萄球菌耐药基因及致病毒素基因的研究

Study on Resistant Gene of Methicillin-resistant and Pathogenic Genes Staphylococcus aureus

目的研究金黄色葡萄球菌耐药基因及致病因子中毒休克综合征毒素-1（TSST-1）基因和杀白细胞毒素（PVL）基因的分布特征及致病性。方法收集临床分离的80株金黄色葡萄球菌，PCR法检测毒素基因TSST-1，PVL和mecA耐药基因。结果80株金黄色葡萄球菌经PCR法，mecA基因检出率为56．3％（45／80），其中2006年为65．8％（25／38），2007年为47．6％（20／42），2007年的mecA基因检出率低于2006年。PVL^＋菌株的分离率为30．3％，PVL阳性的耐甲氧西林金黄色葡萄球菌（MRSA）为13株（13／45，28．9％），金黄色葡萄球菌（MSSA）为11株（11／35，31．4％），差异无统计学意义（P〉0．05）。TSST-1基因检出率为6．3％（5／80），MSSA中未检出TSST-1基因。结论产PVL和TSST-1的MRSA致病力更强，增加了临床治疗的难度，对MRSA引起的感染应加强控制，防止其在医院播散流行。

Objective To investigate antibiotic resistant gane of methicillin-resistant Staphylococcus aureus and the distribution characteristics of panton-valentine leukkocidin （PVL）, toxic shock syndrome toxin （TSST-1）of methicillin-resistant staphylococcus aureus（MRSA）. Methods 80 Staphylococcus aureus were collected from the hospital. The detection of PVL gene,mecA gene and TSST-1 gene were carried out by PCR. Results Identified PVL gene in 24 isolates. The prevalence of PVL was 30. 3% in S. aureus isolates. The prevalence of PVL gene were 28.9% in MRSA and 31.4% in MSSA. The dif- ference was not statistically significant（P^0. 05）. The average rate of meeA gene in 80 Staphylococcus aureus was 56.3% （45/80）. The average rate of mecA gene was 65. 8%（25/38） in 2006 and 47. 6%（20/42） in 2007. The average rate of mecA gene in 2007 was lower than in 2006. The prevalence of TSST-1 gene was 6.3% and TSST-1 gene was not detected in MSSA. Conclusion MRSA which produced PVL,TSST-1 possess more pathogenic power so that it brings difficulty to clinic treatment. Measures should be taken to control its spread.