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The Interaction of Spinach Nitrite Reductase with Ferredoxin： A Site-Directed Mutation Study 被引量：1

The Interaction of Spinach Nitrite Reductase with Ferredoxin： A Site-Directed Mutation Study
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摘要 A series of site-directed mutants of the ferredoxin-dependent spinach nitrite reductase has been characterized and several amino acids have been identified that appear to be involved in the interaction of the enzyme with ferredoxin. In a complementary study, binding constants to nitrite reductase and steady-state kinetic parameters of site-directed mutants of ferredoxin were determined in an attempt to identify ferredoxin amino acids involved in the interaction with nitrite reductase. The results have been interpreted in terms of an in-silico docking model for the 1：1 complex of ferredoxin with nitrite reductase. A series of site-directed mutants of the ferredoxin-dependent spinach nitrite reductase has been characterized and several amino acids have been identified that appear to be involved in the interaction of the enzyme with ferredoxin. In a complementary study, binding constants to nitrite reductase and steady-state kinetic parameters of site-directed mutants of ferredoxin were determined in an attempt to identify ferredoxin amino acids involved in the interaction with nitrite reductase. The results have been interpreted in terms of an in-silico docking model for the 1：1 complex of ferredoxin with nitrite reductase.
作者 Masakazu Hirasawa Jatindra N. Tripathy Ramasamy Somasundaram Michael K. Johnson Megha Bhalla James R Allen David B. Knaff
机构地区 Department of Chemistry and Biochemistry Department of Chemistry Department of Chemistry and Biochemistry Institute for Biotechnology and Genomics
出处 《Molecular Plant》 SCIE CAS CSCD 2009年第3期407-415,共9页 分子植物（英文版）
关键词 Electron transport ENZYMOLOGY nitrogen metabolism molecular biology. Electron transport enzymology nitrogen metabolism molecular biology.
分类号 Q943 [生物学—植物学] S636.1 [农业科学—蔬菜学]
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Molecular Plant
2009年 第3期

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