重组人甲状旁腺素(1-34)对角质形成细胞分泌IL-6和IL-8的影响

Effect of Recombinant Human Parathormone(1-34) on the Excretion of IL-6 and IL-8 by Keratinocyte

目的研究人甲状旁腺素(PTH)(1-34)对角质形成细胞分泌IL-6和IL-8的影响,探讨其治疗银屑病的分子机理。方法体外培养人角质形成细胞株(HaCaT),研究中设空白对照组、骨化三醇组、骨化三醇和PTH(1-34)联合组以及1.0×10-7mol/L～1.0×10-10mol/L4个不同剂量的PTH(1-34)给药组。48h后收集上清液,用酶联免疫吸附法(ELISA)检测HaCaT细胞分泌至培养液中IL-6和IL-8的含量。结果在1.0×10-7mol/L～1.010-10mol/L四个梯度浓度PTH(1-34)及联合骨化三醇处理48h后,HaCaT细胞分泌IL-6量和空白对照组、骨化三醇组比较,差异无统计学意义,而IL-8的分泌量明显降低(P<0.05),且1.0×10-7mol/LPTH(1-34)处理组IL-8分泌量明显低于其他各组。结论PTH(1-34)治疗银屑病的作用机制可能部分通过调节IL-8的表达和分泌来实现。

Objective To investigate the effect of recombinant human parathormone（1-34） on the excretion of IL-6 and IL-8 by keratinocyte and the molecular mechanism underlying its therapeutic effect on psoriasis. Methods Human keratinoeyte was cultured in vitro for investigation. Cultured cells were divided into 7 groups ： （ 1 ） control group ; （ 2 ） ealeitriol group ; （ 3 ） calcitriol ＋ PTH（1-34） group; （4）four PTH（1-34） groups with different PTH（1-34） dosages ranging from 10^-7mol/L to 10^-10mol/L. Supernatant was collected 48h after treatment, and ELISA was used to detect the expression level of IL-6 and IL-8. Results The amount of IL-6 in four PTH （ 1-34 ） groups or caleitriol ＋ PTH （1-34） group was of no significantly different that of comparing with control or caleitriol group. However, the amount of IL-8 in four PTH（1-34） groups and caleitriol ＋ PTH（1-34） group was significantly lower than that of control or ealcitriol group （ P 〈 0.05 ）. Conclusion The therapeutic effect of PTH （ 1-34 ） on psoriasis may partly through modulating the expression and excretion of IL-8.