慢性阻塞性肺疾病患者淋巴细胞颗粒溶素的表达及其与血清γ，干扰素、白介素2相关性研究

Expression of granulysin in iymphocytes and correlation analysis with interferon-γ interleukin-2 serum concentrations in chronic obstructive pulmonary disease patients

目的探讨慢性阻塞性肺疾病（chronic obstructive pulmonary disease，COPD）患者不同病期颗粒溶素表达特点以及和γ干扰素（interferon-γ，IFN-γ）、白介素2（interleukin-2，IL-2）之间的关系。方法采用免疫细胞化学技术检测108例COPD患者及46名健康对照组外周血淋巴细胞的表达水平，采用双抗体夹心ABC—ELISA法同步测定血清IFN-γ、IL-2浓度。结果颗粒溶素在COPD患者和健康对照组外周血淋巴细胞中的阳性表达率比较差异有统计学意义（P〈0．01）。颗粒溶素在健康对照组、临床缓解期、急性发作期的外周血淋巴细胞中表达比较差异有统计学意义（P〈0．01）。COPD患者急性发作期血清IFN-γ、1L-2浓度较临床缓解期和健康对照组显著增高（P〈0．01）。COPD患者急性发作期和临床缓解期颗粒溶素表达与血清IFN-γ、IL-2浓度变化均呈正相关，并随着病情变化存在明显消长关系。结论颗粒溶素参与了COPD炎症过程，起着清除病原体的积极作用。IFN-γ、IL-2与颗粒溶素在消除病原体方面可能起着相互协同的作用，可能影响颗粒溶素的释放。

Objective To detect the expression of granulysin in peripheral blood [ymphocytes and its correlation analysis with interferon-γ （ IFN-γ ）, interleukin-2 （ IL-2 ） serum concentrations in the immunopathological process of chronic obstructive pulmonary disease（COPD）. Methods The expression of granulysin in peripheral blood lymphocytes from 108 patients with COPD and 46 healthy controls was evaluated by means of immunocytocbemieal analysis. Meantime, the concentrations of IFN-T and IL-2 in serum from these patients and healthy controls were also measured by means of double antibody sandwich ABC-ELISA. Results Compared with control subjects,patients with COPD showed an increased expression of granulysin （ P 〈0.01 ）. During stables, compared with exacerbations, the expression of granulysin was reduced（ P 〈0.01）, but compared with controls, it was still higher（ P 〈0.01）. The serum concentrations of IFN-γ,IL 2 in acute exacerbations were significant higher than those in stables and controls（ P 〈0.01）. There were positive correlation between the expression of granulysin in acute exacerbations and stables and the change of IFN-γ, IL-2 serum concentration. Conclusions Granulysin may involve in the process of inflammation in COPD by means of eliminating pathogens. There was positive correlation between the expression of granulysin and IFN-γ, IL-2 serum concentration. IFN-γ and IL-2 may play synergetic roles in the process of eliminating pathogen and play a positive cole in the release of granulysin.