摘要
目的:研究急性白血病(AL)患者MK和VEGF基因的表达水平及其与血管新生、临床预后的关系。方法:应用实时荧光定量PCR检测95例不同病程的AL患者骨髓单个核细胞MK和VEGF mRNA的表达。结果:急性非淋巴细胞白血病(AML)和急性淋巴细胞白血病(ALL)患者MK和VEGF表达量均明显高于对照组,P=0.000 0。初治/复发组AL患者MK、VEGF基因表达水平较对照组及完全缓解组升高,完全缓解组MK和VEGF表达水平仍较对照组高,P均<0.01。缓解组MK表达水平与VEGF和骨髓原幼细胞数存在显著相关性,r=0.673,P<0.01;r=0.468,P<0.01。复发组MK和VEGF之间也存在相关性,r=0.526,P<0.05。初治组和对照组MK、VEGF、骨髓原幼细胞数之间无明显相关性。结论:MK、VEGF高表达可能与AL血管新生、病情进展有关,且两者存在一定的协同作用,共同监测可能对研究急性白血病的发病及指导治疗和估计预后有一定意义。
OBJECTIVE.. To explore the expression and correlation of MK and VEGF gene in acute leukemia （AL）, and evaluate their relationship with angiogenesis and prognosis. METHODS.. The expression level of MK and VEGF in 95 AL patients in different phase were detected by Taqman real-time fluorescent quantitative RT PCR. RESULTS： The expressions level of MK and VEGF in acute myelogenous leukemia （AML） and acute lymphoblastic leukemia （ALL） were significantly higher than those in control group （P=0. 000 0） ; The expression level of MK and VEGF in untreated/ relapsed groups was significantly higher than that in control/remission groups （P〈0.01）; The expression level of MK and VEGF in remission group was also significantly higher than that in control group（P〈0.01）. Linear correlation was observed between the level of MK and VEGF in relapsed group（r=0. 526,P〈0.05）. In remission group, MK had linear relationship with VEGF and the ratio of the blast cell in bone marrow（r= 0. 673,P〈0.01; r= 0. 468,P〈 0.01 ）, but in untreated/control groups, there was no linear relationship among MK, VEGF level and the ratio of the blast cell in bone marrow. CONCLUSIONS.. The high expressions of MK and VEGF are correlated with angiogenesis and prognosis in AL, and there is synergistic effect between MK and VEGF. Monitoring MK and VEGF can be used to guide the treatment and to estimate prognosis.
出处
《中华肿瘤防治杂志》
CAS
2009年第9期690-693,共4页
Chinese Journal of Cancer Prevention and Treatment
