口服高渗氯化钠维生素C液对腹腔感染大鼠肠屏障的保护作用

Influences of oral hypertonic saline with vitamin C on gut barrier function of rats with abdominal infection

目的:探讨口服高渗氯化钠维生素C液对腹腔感染的治疗作用。方法:选择雄性wister大鼠60只,随机分为4组,各组均行盲肠结扎穿孔手术造模,术后分别灌以生理盐水、高渗氯化钠液、维生素C、高渗氯化钠维生素C液。采集门静脉血测定内毒素含量,取回肠末段行病理学观察,并测定肠组织MDA、SOD、羟自由基的含量。结果:高渗氯化钠维生素C液治疗组疗效最好。含维生素C治疗液组(VC组与HSVC组)与不含维生素C成分组(HNS组和NS组)相比SOD活性显著提高,具有统计学意义(P<0.05)。含高渗氯化钠成分治疗液组(HSVC组和HNS组)与不含有高渗氯化钠成分的组(VC组和NS组)相比抑制羟自由基的能力显著提高,具有统计学意义(P<0.05)。结论:高渗氯化钠维生素C对腹腔感染大鼠肠屏障有较明显的保护作用。

Objective： Free radicals are involved in the pathogenesis of intestinal barrier injury in abdominal infection. We investigated the effects of hypertonic saline combined with/without vitamins C on intestinal oxidative stress injury of abdominal infection in rats. Methods： 60 adult wister rats were randomly divided into 4 groups： 0.9% saline group （NS ,n=15）,10% hypertonic saline group（HS, n=15）, vitamins C group （VC, n=15） and hypertonic saline with vitamins C group（HSVC ,n =15）. Cecal ligation plus puncture （CLP） was performed to induce sepsis in all rats. Blood samples were collected from the portal vein of rats for assessment of endotoxin level. The ileum was sampled for pathological change examination and assessment of malonaldehyde, superoxide dismutase, the ability of hydroxy radical inhibition 48h after operation. Results： The ileum SOD level of vitamin C contained group （VC＋HNVC） is higher than non-vitamin C group （NS＋HNS, P〈0.05）and the ability of hydroxy radical inhibition of hypertonic group （HNS＋HNVC） is significantly increased than isotonic group （NS＋VC, P 〈0.05）.The pathological evaluation of HSVC group is better than other groups. Conclusion： There are synergistic antioxidative effects among the antioxidants, hypertonic saline and vitamins C, which protect the intestinal barrier from oxidative stress injury of abdominal infection in rats, suggesting that antioxidant treatment may best be done using a balanced ＂cocktail＂.