摘要
分子标记技术近年来发展很快,目前,常用的分子标记主要可以分为基于杂交的分子标记、基于PCR的分子标记、基于限制性酶切和PCR结合的分子标记以及新一代分子标记。本文对这几类分子标记中常用类型的基本原理进行介绍,并从分子标记在作物种质遗传多样性、基因定位和基因克隆以及分子标记辅助选择育种和分子设计育种中应用进行了阐述。
The technique of molecular markers was developed very quickly. At present, the molecular markers could be divided into four types, i.e., the molecular markers based on hybridization, PCR, the combination of restriction enzyme digestion and new typed molecular markers. In the present paper, we introduced the principal of those types of molecular markers, and described the utilization on genetic diversity ofgermplasm, genetic mapping and cloning, molecular assisted selection and molecular design breeding.
出处
《现代生物医学进展》
CAS
2009年第11期2179-2181,2148,共4页
Progress in Modern Biomedicine
关键词
分子标记
遗传育种
遗传多样性
辅助选择育种
分子设计育种
Molecular markers
Genetic breeding
Genetic diversity
Molecular assisted selection
Molecular design breeding