1个烟草糖基转移酶启动子在拟南芥中的表达

Expression of a Tobacco Glycosyltransferase Gene's Promoter in Arabidopsis thaliana

从烟草中克隆到1个受甲基茉莉酸和水杨酸双重诱导的糖基转移酶基因GT-MS,将其翻译起始位点5′上游-800^+1启动子序列取代pCAMBIA1301质粒的35S启动子,构建GT-MS启动子与Gus基因融合的植物表达载体,采用花序浸染法转化拟南芥,得到阳性植株。对转基因植株进行组织化学染色,结果表明,GT-MS启动子在拟南芥不同生长发育时期及不同器官均有表达,但主要是在维管组织表达,且韧皮部的表达高于木质部。MeJA和SA诱导后GUS活性增强,Gus基因RNA表达量成倍增加。表明GT-MS启动子与在烟草中相似,在拟南芥中也受MeJA和SA诱导。

A glycosyltransferase gene （GT-MS） induced both by methyl jasmonate and salicylic acid was cloned from Tobacco. The fusion vector of the GT-MS promoter （5＇-800-＋1） and Gus gene within pCAMBIA1301 was constructed and transferred to Arabidopsis thaliana through dipping flower. The results of histochemieal staining of GUS activity showed that the GT-MS promoter expressed at different stage of growth and development and in different organs of transgenic plants,but mainly in the canaliculus. The expession level in phloem is higher than that in xylem. GUS activity and Gus RNA expression were increased after the leaves were treated with methyl jasmonate and salicylic. These results showed that the GT-MS promoter was also induced in Arabidopsis thaliana by MeJA and SA,as it was in tobacco.