嗅球提取液脑室内注射对大鼠阿尔茨海默病的影响

Effects of distilled fluid of olfactory ball by intracerobreventricular injection on treatment of Alzheimer′s disease rat

目的探讨嗅球提取液（DFOB）脑室内注射对大鼠阿尔茨海默病（AD）的影响。方法SD大鼠双侧海马注射Aβ1-40,建立AD大鼠模型。实验动物分为4组：健康对照组、AD模型组、人工脑脊液（MCSF）注射组、DFOB注射组，每组8只。运用行为学测试、NADPH—d组织化学、刚果红组织学染色结合积分吸光度测定等技术，观察、比较各组AD模型大鼠注射4周后学习记忆能力、一氧化氮合酶（NOs）阳性神经元数以及老年斑的变化。结果DFOB注射组大鼠达到学会标准所需训练次数、错误反应次数、潜伏期和全天总反应时间均小于AD模型组、MCSF注射组（P〈0．05）。DFOB注射组NOS阳性神经元数较AD模型组、MCSF注射组明显增多（P〈0．05）。DFOB注射组刚果红染色及其吸光度测定与AD模型组、MCSF注射组比较差异有统计学意义。MCSF注射组与AD模型组差异均无统计学意义。结论DFOB脑室内注射对大鼠AD具有明显的治疗作用。

Objective To probe the effects of distilled fluid of olfactory ball （DFOB） by intracerebroventricular injection on treatment of Alzheimer＇s Disease （AD） rat. Methods The model of AD rats were built by intracerebroventricular injection of Aβ1-40. Four weeks after being injected, the change of memory recovery was observed by Morris-labyrinth test, the total number of nitric oxide synthase positive neurons and senile plaques of hippocampus were observed by NADPH-d and Congo red histochemical staining. Results TN, EN, D, TRT of DFOB injected group obviously decreased compared to the model of AD group and MCSF injected group （P〈 0.05）. The total number of NOS positive neurons of DFOB injected group were higher than that of the model of AD group and MCSF injected group （P〈0.05）. Congo red histochemical staining： compared to the model of AD group and MCSF injected group, the latter was significantly changed in SP- IA （P〈0. 05）. There was not significant differences between the model of AD group and MCSF injected group（P〉0.05）. Conclusion The distilled fluid of olfactory ball can greatly improve the effects on treatment of AD rat by intracerebreventricular injection.